真核生物转录因子及其研究方法进展

Progress on Transcription Factors and Theirs Methods of Study in Eukaryon

真核生物基因的表达调控是当前分子生物学最前沿的研究领域之一,而转录水平的调控是基因表达过程中最重要的第一步,由于蛋白质-蛋白质、蛋白质-DNA之间的相互作用,以及一些复杂大分子复合物的形成,导致真核生物转录水平的调控是一个多级的复杂过程。转录因子即反式作用因子在转录调控中可以直接或间接的识别或结合在顺式作用元件8 bp^112 bp核心序列上,参与调控靶基因的转录效率。因此,转录因子与调控序列的相互作用在决定某个基因是否表达中占据核心位置。随着对转录因子及其研究方法的深入研究,尤其是近几年染色质免疫沉淀及生物信息学的发展,人们将会进一步研究转录调控的机制及细胞行为和疾病的发生机理。

Research in gene expression regulation in eukaryon is one of the leading molecular research fields. Regulation in transcription level is the key first step of gene expression regulation. Due to the interaction between proteins,proteins and DNA,and the formation of some complicated large compounds in the gene transcription regulation, gene transcription regulation is also a very complex progress with multiple steps. Transcription factor can identify or binding to core sequence（8 bp-12 bp） of cis-acting element directly or indirectly, and participate in regular the transcription efficiency. So the interactions between transcription factor and regulating sequence occupies the core position in determining one gene whether can express. As further research abount the transcription factor and its methods, especially the development of chromatin immunoprecipitation and bioinformatics, the mechanism of transcriptional control, the cell behavior and disease＇s occurrence mechanism will be further known.