摘要
目的研究栀子总环烯醚萜苷对大鼠脑出血后炎症反应和神经元凋亡的影响。方法将SD大鼠随机分为假手术组、模型组、清开灵注射液组(3000mg/kg)和栀子总环烯醚萜苷高、中、低剂量组(30,15,7.5mg/kg),采用自体血注入诱发大鼠脑出血模型,在术后48h取脑组织标本,放射免疫法检测血肿周围脑组织TNF-α和IL-1β含量,免疫组化检测ICAM-1和Caspase-3的表达。结果与模型组相比较,栀子总环烯醚萜苷中、高剂量组血肿周围脑组织TNF-α和IL-1β含量明显降低,具有显著性差异(P<0.05或P<0.01),ICAM-1和Caspase-3表达明显下调,差异具有显著性(P<0.05或P<0.01)。结论栀子总环烯醚萜苷可以干预脑出血后炎症反应,阻止神经元凋亡。
Objective To study the effect of total iridoid glycosides from Fructus Gardeniae （TIG - FG） on inflammatory reaction and neuronal apoptosis in rats with intracerebral hemorrhage （ICH）. Methods Male Sprague - Dawley rats were randomly divided into pseudo - operation group, model group, Qingkailing Injection group（3000 mg/kg） and TIG - FG groups（30, 15 and 7.5 mg/kg respectively）. ICH rat models were induced by stereotactic injection of autologous blood （50 μL） into pallidus. The brain tissue specimens in every group were gathered at the time of 48 h after operation. Radioimmunoassay（RIA） was utilized to identify the content of tumor necrosis factor-alpha （TNF-α） and interleukin lbeta（IL - 1β）. The expression of intercellular adhesion molecule （ICAM - 1 ） and Caspase - 3 in rats after ICH was detected with inmunohistochemical method. Results Compared with ICH model group, the contents of TNF-α and IL- 1β in brain tissue of TIG - FG group （ 15 or 30 mg/kg） were decreased （ P 〈 0. 05 or P 〈 0. 01 ）, and the expression of ICAM - 1 and Caspase - 3 was obviously decreased （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Total iridoid glycosides from Fructus gardeniae can counteract the inflammatory reaction and inhibit the neuronal apoptosis in rats brain after intracerebral hemorrhage.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第1期8-10,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家科技部十五攻关西部重点项目(001BA901A45)。
