心复力冲剂对心力衰竭猫血流动力学和大鼠心肌病理形态的影响

Effects of Xinfuli Granula on Hemodynamics of Cardiac Failure Cats and on Myocardial Pathological Feature in Rats

目的探讨心复力冲剂对急、慢性心力衰竭的防治作用。方法以戊巴比妥钠致猫急性心力衰竭,从十二指肠一次性灌入心复力悬液,给药后连续记录血流动力学参数。大鼠随机分为5组,以异丙肾上腺素20mg/kg致大鼠心肌损伤模型,正常组与病理对照组给予生理盐水,阳性对照组静脉注射西地兰0.2mg/kg,给药组给予心复力冲剂或普奈洛尔,在光镜下观察心肌细胞的坏死程度及炎性细胞浸润情况。结果心复力冲剂对巴比妥致急性心力衰竭猫的心脏血流动力学无明显影响;对异丙肾上腺素致大鼠心肌损伤、心肌缺血具有显著的防治作用。经心复力冲剂治疗后,大鼠心肌损伤程度明显减轻,病灶范围减少,以修复反应为主。结论心复力冲剂可对抗异丙肾上腺素所致大鼠的心肌损伤,对实验性亚急性心力衰竭具有显著的保护作用,但对急性心力衰竭无防治作用。

Objective To study the protective effect of Xinfuli Granula （XG） on acute and chronic cardiac failure in cats and rats. Methods The cat models of acute cardiac failure were established by injecting pentobarbital. Then the cat models were given XG through duodenum. After medication, hemodynamics parameters such as heart rate （HR） , mean arterial pressure （MAP）, left ventricular systolic pressure （LVSP）, left ventricular end -diastolic pressure （LVEDP）, ＋ dp/dtmax and cardiac output （CO） in cats were determined. The rats were divided randomly into 5 groups： the control group, the model group, the positive control group, and high - close and Low - dose XG groups. The rat models of myocardial injury were induced by isoproterenol （20 mg/kg）. The normal group and the model group were treated with normal saline （10 mL/kg） orally, the positive eontral group were treated with cedilanid （0.2 mg/kg） by intravenous, the high- and low- dose XG groups were orally treated with XG （10 g/kg and 5 g/kg respectively）, and the propranolol group was orally treated with propranolol （4 mg/kg）, once a day for successive 8 days. Animals were sacrificed on the 9th day, then the heart was taken out and the cardiac index was calculated. The pathological changes in myocardial cells of the rats were observed under electron microscope. Results XG had no effect on hemodynamics of cats with acute cardiac failure, but had obvious protective and therapeutic effect on isoproterenol - inducedmyocardial injury and myocardial ischemia of rats. Conclusion Xinfuli Granula has good preventive effect on isoproterenol -induced myocardial injury and sub - acute cardiac failure of rats, but has no effect on acute cardiac failure of cats.