间充质干细胞诱导体外分化来的初始T细胞表型变化的实验研究

Induction of phenotype variance of naive T cell derived in vitro from mesenchymal stem cells

目的将体外由CD34^＋细胞分化而来的初始T细胞与异基因骨髓间充质干细胞（MSC）共孵育，观察初始T细胞表型的变化。方法传3代以上的MSC与脐血CD34^＋刍细胞分化的初始T细胞共孵育1周，用流式细胞仪检测其表型变化。结果与对照组相比，CD8^＋细胞明显增加（共孵育组（35．9±6．3）％，单纯初始T细胞培养组（18．4±4．5）％]，且CD8^＋；CD3^＋细胞明显增加[共孵育组（27．6±2．8）％，单纯初始T细胞培养组（15．2±3．1）％]。结论骨髓MSC在体外与异基因初始T淋巴细胞共孵育使CD8^＋初始T细胞表达增加，这种变化可能与其诱导免疫耐受相关。

Objective Donor derived naive T cells initiated GVHD by contacting with, mesenchymal stem cells （MSC） have been used to prevent or treat graft-versus-host disease （GVHD）. although we still puzzle about its mechanisms. Observe the effect of MSC on phenotypes of Naive T cell to study the mechanism of MSC immunomodulation. Methods After 3 passages, MSC was incubated with Naive T cell differentiated from cord blood CD^34＋ cells in vitro. Then the variances of naive T cell phenotypes were analyzed by flow cytometric. Results CD8^＋ T cells were relatively increased after 7 days co-culture with allogenie MSC when compared to control： （35.9±6.3）% vs （18.4±4.5）%. CD8^＋ CD; cells also showed the same trend （27.6±2.8）% vs （15.2±3.1）%. Conclusion MSC may partly reduce the incidence of GVHD by increase of CD8^＋ naive T cell. The result may provide new clue to explain immunoregulatory mechanism of MSC.