丙戊酸钠对Kasumi-1细胞周期蛋白及周期蛋白依赖性激酶抑制剂的影响

The study of influence on cyclin and cyclin-dependent kinase inhibitor of Kasumi-1 cell treated with VPA

目的探讨组蛋白脱乙酰化酶（HDAC）抑制剂丙戊酸钠（VPA）对t（8；21）／AML细胞株Kasumi-1细胞周期蛋白及其周期蛋白依赖性激酶抑制剂基因的影响。方法将Kasumi-1细胞经不同浓度VPA处理不同时间，应用反转录聚合酶链反应（RT—PCR）技术检测细胞周期调节因子mRNA水平的变化。结果VPA下调cyclinD1、cyclinE1及cyclinB1 mRNA水平，上调p21^WAF1/CIP1 mRNA表达，对p27^KIP1 mRNA表达无明显影响。结论VPA可通过对Kasumi-1细胞周期蛋白及周期蛋白依赖性激酶抑制剂的影响调节细胞周期，使细胞阻滞于G0／G1期。

Objective To explore the influence on cyclin and cyclin-dependent kinase inhibitor of cell cycle of Kasumi-1 cell-derived from t（8;21）/AML treated with histone deacetylase （HDAC）inhibitor valproic acid（VPA）. Methods RT-PCR assay was applied to detect mRNA expression of regulation factor of cell cycle of Kasumi-1 cell with VPA at different concentration and different time. Results VPA could down-regulate mRNA expression of cyclinD1, cyclinE1 and cyclinB1, and up-regulate mRNA expression of p21^WAF1/CIP1 with no obvious variation of mRNA level of p27^KIP1. Conclusion VPA could regulate cell cycle through the regulation of cyclins and cyclin-dependent kinase,arresting Kasumi-1 cell at G0/G1 phase.