多发性骨髓瘤患者外周血T细胞中T细胞受体ξ链基因表达特点

The feature of TCR ξ chain gene expression in peripheral blood T cells from patients with multiple myeloma

目的分析多发性骨髓瘤（MM）患者外周血T细胞中T细胞受体（TCR）ξ链基因表达水平，了解患者T细胞活化过程的相关变化情况。方法采用SYBR Green Ⅰ相对定量PCR方法分别检测24例MM患者和24名健康人的外周血单个核细胞（PBMC）的TCRξ链基因表达情况，以β2微球蛋白基因（β2M）作为内参照，根据相对定量公式（2^-△Ω×100％）分别计算TCRξ链基因表达量。结果MM患者外周血TCRξ链基因表达水平为（1．83±1．72）％，低于健康人的（3．46±2．75）％（P=0．019）。TCRξ链基因表达水平的变化与患者年龄无相关性（r=0．151，P=0．525）。结论首先报道MM患者TCRξ链表达的变化特点，TCRξ链基因表达水平以降低为主，可能与T细胞免疫缺陷有关。

Objective To investigate T-cell receptor （TCR）ξ chain gene expression level in peripheral blood T cells from patients with muhiple myeloma （MM）, thereby to estimate the feature of T cells activation status. Methods Real-time PCR with SYBR Green Ⅰ technique was used for detecting TCR chain expression level in peripheral blood mononuclear cells （PBMC） of 24 cases with MM and 24 normal individuals. β2 -microglobulin （β2M） gene expression was used as an endogenous reference. Relative changes in TCR ξ chain expression level were analyzed by the 2^-△Ω×100% method between patients with MM and normal individuals. Results Compared with normal individuals, TCR ξ chain gene expression was obviously down regulated in PBMC from patients with MM （P =0.019）. The expression level of TCR ξ chain gene is not significantly age-associated in MM patients[（1.83±1.72）%,（3.46±2.75）%]（P =0.525）. Conclusion This is the first description in the expression feature of TCR ξ chain gene in MM patients. TCR ξ chain expression was decreased in most of MM patients, which might be related to celluar immunodeficiency.