血管内皮生长因子及其受体在喉鳞癌中的表达

Study on expression and correlation of vascular endothelial growth factor and its receptors in laryngeal squamous cell carcinoma

目的探讨血管内皮生长因子（VEGF）及其受体Fit-1及KDR在喉鳞癌生长、转移过程中的作用及机制。方法采用Western—blotting法和RT-PCR法检测20例喉鳞癌组织及18例癌旁组织中VEGF、Fit-1及KDR的蛋白和mRNA的表达水平。结果VEGF、Fit—1及KDR蛋白和mRNA在喉鳞癌组织中的表达分别为4．32±2．21、2．00±0．91，1．20±0．55、0．29±0．31，2．50±1．69、0．85±0．28，差异有统计学意义（均P〈0．01）；VEGF、Flt-1及KDR蛋白和mRNA在颈淋巴结转移组与非颈淋巴结转移组差异有统计学意义（P〈0．01或P〈0．05）；VEGF与KDR在喉鳞癌及癌旁组织中的表达水平之间呈正相关（P〈0．01），而VEGF与Flt-1在喉鳞癌组织中的表达水平之间则无相关性（P〉0．05）。结论VEGF及其受体Flt-1、KDR参与了喉鳞癌新生血管增生过程，与喉鳞癌的生长、浸润和淋巴结转移密切相关，KDR是喉鳞癌组织中VEGF发挥作用的重要受体。

Objective To clarity the role of VEGF and its receptors in laryngeal squamous cell carcinoma development and metastasis. Methods The protein and mRNA expression of VEGF and its receptors （Flt-1 and KDR） were examined by Western-blotting and semiquantitative reverse transcriptase polymerase chain reaction analysis in laryngeal squamouse cell carcinoma （20 specimens） and the adjacent non-neoplastic laryngeal tissues （18 specimens）. Results The protein and mRNA expression levels of VEGF and its receptors（Flt-1 and KDR） were more abundant in laryngeal squamous cell carcinoma than that in the adjacent non-neoplastic laryngeal tissues （orderly 4.32±2.21, 2.00±0.91. 1.20±0.55, 0.29±0.31. 2.50±1.69, 0.85±0.28. P 〈0.01）. The expression of VEGF and its receptors（Flt-1 and KDR） in laryngeal squamous cell carcinoma was significantly higher in lymph node positive group than that in lymph node negative group （P〈 0.01 or P 〈0.05）. Positive correlation was observed between VEGF and KDR in the laryngeal squamouse cell carcinoma and adjacent non-neoplastic laryngeal tissues （P 〈0.01）. There was no correlation between VEGF and Flt-1 expression（P 〉0.05）. Conclusion The intense expression of VEGF, Flt-1 and KDR in laryngeal squamous cell carcinoma provides strong evidence linking VEGF, Flt-1 and KDR expression to the angiogenesis associated with laryngeal squamous cell carcinoma. The positive correlation between VEGF expression and KDR expression clearly demonstrates that KDR is the main signaling receptor for VEGF in laryngeal squamouse cell carcinoma. The VEGF/KDR system plays a functional role in the progression of laryngeal squamous cell carcinoma.