uPAR和VEGF—C在原发性肝细胞癌中的表达及其临床意义

Expression and significance of uPAR and VEGF-C in hepatocellular carcinoma

目的探讨尿激酶型纤溶酶原激活物受体（uPAR）和血管内皮生长因子-C（VEGF—C）在原发性肝细胞癌中的表达及其与肝癌部分生物学行为的关系。方法采用常规sP免疫组织化学染色法检测33例原发性肝细胞癌标本中uPAR和VEGF—C的表达，分析其表达水平与肝癌部分生物学行为的关系。结果uPAR和VEGF—C定位于肝癌细胞细胞质，在33例原发性肝细胞癌中，uPAR阳性表达率为69．7％（23／33），VEGF—C阳性表达率为78．8％（26／33）。肝癌转移组的uPAR和VEGF—C阳性表达率均明显高于无转移组，二者比较差异均有统计学意义（P〈0．05），但它们的表达与组织学分级、肿瘤大小、是否伴有肝硬化、AFP表达等均无相关性。uPAR和VEGF—C在肝癌组织中的阳性表达呈正相关（r=0．676，P〈0．01）。结论uPAR和VEGF—C的表达与肿瘤侵袭、转移的发生有关，可作为估计预后的指标，对判断肝癌的恶性程度，预测及生物学行为具有一定的临床意义。

Objective To investigate the expression of uPAR and VEGF-C in hepatocellular carcinoma （HCC） and its effect on the biological behavior of HCC. Methods The expression of uPAR and VEGF-C in liver tissue specimen of 33 patients with HCC was detected by immunohistoehemieal staining. Results The positive expression of uPAR and VEGF-C in HCC tissues was located in cytoplasma of cancer cells. The positive rates of uPAR and VEGF-C were 69.7 %（23/33） and 78.8 %（26/33） respectively in 33 cases of HCC. The expression rates of uPAR and VEGF-C were significantly higher in HCC with metastasis than in HCC without metastasis（P 〈0.05）. But there were not significant correlation between the expression of uPAR or VEGF-C and tumor size, tumor grade, AFP level, hepatic cirrhosis. There was a close correlation between uPAR and VEGF-C in HCC（r =0.676,P 〈0.01）. Conclusion The expression of uPAR and VEGF-C is correlated with metastasis, uPAR and VEGF-C may be signs of aggressive phenotype for evaluating treatment and prognosis of HCC. Examination of these two proteins may be helpful to judge biological behavior of HCC.