大黄素对牙周炎大鼠牙周组织中骨钙素白细胞介素6表达的影响

Effect of emodin on level of bone gla protein and cytokine interleukin-6 in periodontium of rats

目的通过观察牙周炎大鼠牙周组织中骨钙素(bone gla protein,BGP)、白细胞介素6(cytokine interleukin-6,IL-6)水平的变化,来探讨大黄素治疗牙周炎的机制。方法选用100只体重160~200 g Wistar大白鼠,随机分成10组,每组10只。A组:正常对照组。B^J组建立大鼠牙周炎模型。B组:牙周炎对照组。B组在牙周炎造模成功后即与A组一同处死。C、D、E、F组分别于牙周炎模型建立后第1、2、3、4周处死。G、H、I、J组均为大黄素治疗组,分别于治疗后第1、2、3、4周处死,与C^F组形成平行对照。大鼠处死后切取上颌牙周组织块制作标本,评价牙周组织的变化,免疫组织化学方法检测牙周组织中BGP、IL-6的表达。结果大黄素治疗组大鼠牙周组织中BGP的含量在实验的各阶段均高于对照组(P<0.05),IL-6的含量在大黄素治疗第3周后开始低于对照组(P<0.05)。结论大黄素具有促进牙周组织形成、抑制炎性反应的作用,对牙周炎的治疗具有重要意义。

Objective To observe the effect of emodin on level of bone gla protein and cytokine interleukin - 6 in periodontium during experimental periodontitis for its treating mechanism. Methods One hundred 160 -200 g Wistar rats were randomized into ten groups,with 10 rats in each group. Group A was used as the normal control. Group B -J were experimental periodontitis model. The rats in group A and B were killed immediately after periodontitis model were established. Group C, D, E and F as the control groups,were fed alcohol as placebo and killed at 1,2,3 and 4 weeks respectively. Group G,H,I and J received the therapy of emodin and were killed at comparable time with group C - F. The level of BGP and IL - 6 in the periodontium were measured with immunohistochemistry after the rats were killed. Results The level of BGP in treated group were much higher （P 〈 0.05）than those in the control groups. The levels of IL- 6 in group I and J were much lower（ P 〈 0.05 ） than those in the control group. Conclusions Emodin promoted the regeneration of the periodontal tissues and inhibited the progress of experimental periodontitis. Emodin could play an important role in treating periodontitis.