低聚核苷酸d(GGTATACC)_2的~1H和^(13)CNMR研究

1H AND 13 C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC) 2 IN SOLUTION

通过综合分析２Ｄ１ＨＮＯＥ谱（混合时间１００ｍｓ），１３Ｃ－１Ｈ异核多量子化学位移相关谱和１Ｈ双量子滤波化学位移相关谱归属了低聚脱氧核糖核酸片断ｄ（ＧＧＴＡＴＡＣＣ）２１Ｈ和１３Ｃ的化学位移．该８聚核苷酸在溶液中为双螺旋Ｂ型结构，各碱基相对于糖环呈反式构象，糖环本身则均以Ｃ２′－ｅｎｄｏ构象为主．

Deoxyoligonucleotide d(GGTATACC) 2 was studied by two dimensional NMR techniques. Its proton and 13 C chemical shifts were assigned by 2D proton NOE spectrum and 13 C- 1H heteronuclear multiquantum chemical shift correlated spectrum (HMQC) combined with double quantum filtered chemical shift correlated spectrum (DQF-COSY). The sugar puckers of the eight residues of this helix octamer were determined to be dominantly C2′-endo forms with anti glycosidic conformations.