大黄素配伍中药活性成分体外抗柯萨奇B_3病毒试验

Experimental Study on the Antiviral Action of Emodin in Combination with Other Chinese Active Medicine Components on Coxsackie Virus B_3 Replication

目的:研究大黄素与不同中药活性成分配伍后体外抗柯萨奇病毒B3(CVB3)的作用,并探讨其抗病毒机制。方法:用不同组成、不同浓度的中药材提取液处理CVB3感染的Hep-2细胞,72h后用MTT法测定细胞活性。结果:在Hep-2细胞系中,所有经配伍后的中药组合都能抑制CVB3致细胞病变作用,提高细胞存活率,降低细胞毒性。对病毒直接作用效力最高的药物组合为大黄素:姜黄素:苦参碱=2:1:2;抗病毒合成效力最高的药物组合为大黄素:姜黄素:苦参碱=2:0:1。结论:大黄素与中药活性成分配伍,其细胞毒性降低,抗CVB3效力增强。

OBJECTIVE： To investigate the antiviral action of emodin in combination with other Chinese active medicine components on Coxsackie virus B3（CVB3） and explore the possible antiviral mechanism.METHODS： Hep-2 cells infected by CVB3 were cultured with different concentration and proportion of the emodin and other Chinese medicine components for 72 hours and their inhibitory effects on CVBa replication were evaluated with cell survival rates by MTT assay.RESULTS： In the Hep - 2 cell system, all the combinations between emodin and other medicine components could inhibit cytopathic effect（CPE） of CVB3-infected cells, increase cells＇ survival rates and decrease the eytotoxicity.The drug combination which showed the best effect of killing viruses directly was emodin ： curcumine ： banlangen（2 ： 1 ： 2）, and the one showing the highest efficacy in inhibiting the replication of CVB3 in cells was emodin ： curcumine ： banlangen（2 ： 0 ： 1） .CONCLUSION： The toxicity of emodin was reduced and its anti CVB3 activity was enhanced by its combined use with other Chinese active medicine components.