DiaionHP-20富集纯化南山茶种子抗原发Ⅰ型骨质疏松有效部位的工艺研究

Purification of the Anti-PMOP Effective Fraction from Camellia semiserrata Seed with DiaionHP-20 Macroporous Adsorption Resin

目的:研究大孔吸附树脂DiaionHP-20富集纯化南山茶种子抗原发I型骨质疏松有效部位的工艺条件。方法:以南山茶种子中总黄酮和多酚的洗脱率和纯度为指标,考察DiaionHP-20的吸附量、载样量、乙醇洗脱浓度、乙醇洗脱用量等参数。结果:DiaionHP-20的吸附量总黄酮为59.5mg.g-1,多酚为23.9mg.g-1。最佳工艺是药液上样量为10.5倍树脂重量,使用3倍树脂质量的蒸馏水洗去水溶性杂质,13.5倍树脂质量的25%乙醇洗脱总黄酮和多酚。结论:Diaion HP-20能够很好地纯化南山茶种子中总黄酮和多酚,工艺简单,成本低,适于规模化生产。

OBJECTIVE ： To study the technical conditions and parameters of DiaionHP - 20 macroporous adsorption resin for the purification of the anti - PMOP（primary Type I osteoporosis） active fraction from Camellia semiserrata seed. METHODS： With the elution ratios and purity of total flavonoids and polyphenol from Camellia semiserrata as indexes, the technical parameters of DiaionHP-20 macroporous adsorption resin including the adsorption quantity, the loading sample, the elution concentration and volume of alcohol were investigated. RESULTS： The adsorption quantity of DiaionHP - 20 macroporous resin on total flavonoids and polyphenol were 59.5mg · g^-1 and 23.9 mg · g^-1 respectively. The optimal technical conditions were as follows：the quantity of drug was 10.5 times that of resin; water-soluble foreign substance was washed off with distilled water weighing 3 times the weight of resin, and total flavonoids and polyphenol were eluted using 25% ethanol weighing 13.5 times the weight of resin. CONCLUSION：DiaionHP -20 macroporous resin had good purification effects on total flavonoids and polyphenol from Camellia semiserrata seeds.The process is simple, low cost, and suitable for lavge scale production.