醒脑开窍针刺法对缺血性中风模型大鼠基底节14-3-3tau的影响

The Effect of Acupuncture Method Named Waking up Mind from Unconsciousness on 14-3-3 Protein Expression in Ischemic Apoplexy Rat Model's Basal Ganglia

目的:观察醒脑开窍针刺法对缺血性中风模型大鼠基底节14-3-3tau蛋白表达水平的影响。方法:成年雄性Wister大鼠随机分为醒脑开窍组、针刺对照组、假手术组、模型组,每组分为6h、24h两个时间段;除假手术组不插入线栓之外,其余各组均采用脑膜中动脉阻塞法复制缺血性中风大鼠模型,清醒之后经行为学评分合格者,醒脑开窍组选用人中穴、内关穴,针刺对照组选用曲池穴、足三里穴各针刺1次,6h组在处死之前10min再针刺1次,24h组在12h、处死前10min时分别针刺1次;各组在规定时间取缺血侧基底节,用Western-blot对结果进行分析。结果:各组针刺前后、各个时间段之间的14-3-3tau表达水平并无显著性差异。结论:①14-3-3tau在缺血性中风中也许并不参与神经的降解过程;②14-3-3tau参与缺血性中风中的神经损伤过程,表达部位不在基底节。③在缺血性中风的6h、24h时间段的病理过程中,14-3-3tau蛋白参与此病理过程,但是表达水平很低,需要进一步的手段才能进行检测。

Objective：To observe the effect of acupuncture method named waking up mind from unconsciousness on 14-3-3 protein expression in ischemic apoplexy rat model＇s basal ganglia. Method：40 Wister male adult rats were divided into four groups randomly, which were group of waking up mind from unconsciousness, acupuncture control group, sham operated group and model group. In every group 6h and 24h time sections were set up. Except sham operated group, the other groups were made into ischemic apoplexy rat models by blocking arteriae meningea media. After waking, ethology was chosen to evaluate them if qualified. Then dart Renzhong and Neiguan in group of waking up mind from unconsciousness, and do Quchi and Zusanli in acupuncture control group. In 6 h time section do acupuncture again before 10 minutes of putting to death,and in 24 h time section do acupuncture at 12 h and before 10 minutes of putting to death. At last basal ganglia in ischamia side was picked up at oriented time, and homogenated. Analyze the extracted protein with Western-blot. Result： There was no difference in 14-3-3 protein expression between the groups. Conclusion ：（1）Perhaps 14-3-3 protein hasn＇t participated in nerval degradating process in ischemic apoplexy. （2）14-3-3 protein participates in nerve injury process, but its expression site is not in basal ganglia. （3）During 6 h and 24 h timing pathological course of ischemic apoplexy, 14-3-3 protein takes part in the pathological course, but does little expression, and has to be detected by some further means.