摘要
采用牛肉膏培养基培养,从不同深度的太湖沉积物中共分离得到603个菌落形态不同的菌株,通过数值分析比较了40个形态表征性状,以不加权平均连锁聚类(UPGMA)的方式进行簇群归类,在0.75水平上可分成4个聚类群和51个亚群.从51个亚群中各随机选取一个菌株,测定其16SrDNA部分序列(约540bp)并据此进行分析,表明这些菌株分别属于Firmicutes、Actinobacteria、γ-Proteobacteria这三大类群,与数据库中已知的芽孢杆菌属(Bacillus)、类芽孢杆菌属(Paenibacillus)、微球菌属(Micrococcus)、节杆菌属(Arthrobacter)、迪茨菌属(Dietzia)和假单胞菌属(Pseudomonas)细菌具有很高的相似性.其中迪茨菌属在国内淡水湖泊沉积物中为首次报道.
The sediment samples collected from the different sites of the Lake Taihu, 2006 were cultured on traditional nutrient medium. 603 bacteria strains were separated with 40 different morphological features, and were grouped into 4 clusters and 51 subclusters at the level of 0.75 with numerical classified based on the unweighted pair group method with arithmetic averages (UPGMA) algorithm. Based on the partial sequences of 16SrDNA (540bp) analysis, the bacteria strains sample chosen from each subclusters randomly were ranked into Firmicutes, Actinobacteria and γ-Proteobacteria three groups, and closely related to Bacillus, Paenibacillus, Micrococcus, Arthrobacter, Dietzia and Pseudomonas genera. The dietzia genera was reported in the sediment of freshwater lake in China for the first time.
出处
《湖泊科学》
CAS
CSCD
北大核心
2009年第1期27-35,共9页
Journal of Lake Sciences
基金
国家自然科学基金项目(40573063)资助.
关键词
太湖
沉积物
可培养细菌
数值分类
多样性
Lake Taihu
sediment
culturable bacteria
numerical taxonomy
diversity