改良的异位子宫内膜细胞培养方法

Improved primary culture of human ectopic endometrial cells

目的探讨子宫内膜异位症患者异位内膜细胞原代培养方法，为研究子宫内膜异位症的发病机制提供体外细胞模型。方法通过消化、过滤、沉降等方法，分离培养子宫内膜异位症异位内膜腺上皮细胞和间质细胞，光学显微镜观察离体细胞形态。结果①异位子宫内膜细胞培养的成功率为76．47％（13／17）；②改良的细胞培养方法，即胰蛋白酶-胶原酶共同消化法与胶原酶消化法成功率比较无显著性差异（X2=0．180，P=0．893）；③使用改良的酶消化法消化时间在4．075±0．215分钟，单纯胶原酶法则需14．144±7．624分钟，经比较有统计学意义（t=-13．732，P〈0．05）。结论使用改良的细胞培养方法不仅可以获得充分的细胞，而且可以节省时间和经费。

Objective To explore primary culture method of ectopic endometrial cells, so as to provide an in vitro cytological model of endometriosis for study on pathogenesis of endometriosis. Methods The ectopic endometrial glandular cells and stromal cells were isolated and cultured through digestion, filtration and sedimentation methods. Morphological characters of eutopic and ectopic endometrial cells were observed under optical microscope. Results （1）The success rate of separation and culture of etopic endometrial cells of endometriosis was 76.47% （13/17） ; （2） In the success rate of improved cell culture methods, that is, trypsin-collagenase common digestion, ther was no difference as compared with collagenase digestion method （X2 = 0. 180, P = 0. 893 ） ; （3） The enzyme digestion time of improved enzyme digestion method （ trypsin-collagenase common digestion） was 4. 075 ±0. 215 minutes and that of single collagenase digestion method was 14. 144 ± 7. 624 minutes, and the difference was significant （ t = - 13. 732, P 〈 0.05 ）. Conclusion The improved cell culture method can not only obtain adequate cells, but also save time and cost.