宫颈癌组织Bcl-2基因的表达与HPV感染的关系

Relationship between expression of Bcl - 2 and HPV infection in cervical cancer

目的研究宫颈癌组织Bcl-2基因的表达与人乳头瘤病毒（HPV）感染的关系。方法应用免疫组化PV-9000法对正常宫颈组织38例、各级宫颈上皮内瘤样变（CIN）组织106例、宫颈癌组织66例中Bcl-2基因的表达水平进行检测。应用PCR方法对66例宫颈癌组织中HPV表达进行检测及分型。结果Bcl-2在各宫颈组织中均有表达，宫颈癌组Bcl-2阳性表达率（57．6％），显著高于正常宫颈组（15．8％）和CINI组（19．4％），P均〈0．01；高于CINⅡ组（28．6％），P〈0．05；与CINlI组（60．0％）比较差异无统计学意义，P〉0．05。宫颈癌组织中Bcl-2蛋白表达与HPV感染具有相关性，P〈0．05。Bcl-2蛋白在HPVl6／18阳性宫颈癌组织和HPV其他型别阳性宫颈癌组织中表达差异无统计学意义，P〉0．05。结论Bcl-2蛋白的高表达可能在宫颈癌的发生发展中起重要作用。Bcl-2蛋白的高表达与宫颈癌HPV感染相关，但与HPV型别关系不大。Bcl-2蛋白可能成为宫颈癌诊断和治疗的指标。

Objective To study the relationship between expression of Bcl -2 and HPV infection in cervical cancer. Methods The expression of Bcl -2 was detected by immunohistoehemistry PV -9000 in 38 cases of benign squamous epithelia, 106 cases of cervical intraepithelial neoplasia, 66 cases of cervical carcinoma. The expression of HPV and it＇s tape was detected by PCR in 66 cases of cercival cancer. Results Bcl -2 were expressed in all of examples. The positive rate of Bcl -2 in cervical carcinoma （57. 6% ） was statistically different from benign squamous epithelia （,15.8%） and CINI（ 19. 4% ）, P 〈 0. 01. The positive rate was higher in cervical carcinoma than in CINⅡ（ 28. 6% ）, P 〈0. 05. The positive rate were similar in cervical carcinoma and CINm （60.0%）, P 〉 0.05. The relationship between the expression of Bcl - 2 and HPV infection were significiant, P 〈 0.05. The expression of Bcl - 2 was not different between HPV16/18 and other type of HPV in cervical cancer, P 〉0.05. Conclusion The expression of Bel- 2 may play an important role in cervical carcinoma occurrence and development. The over- expression of Bcl - 2 have signigificiant relationship with HPV infection. But it＇ s expression are not correlated with the type of HPV. Bcl -2 may be a diagnosis and therapy, index of cervical cancer.