摘要
以Columbia型拟南芥为供试材料,对其在不同培养基和不同浓度植物激素配比条件下的出愈率及愈伤组织状态进行研究。结果表明,NB培养基可明显提高拟南芥的愈伤组织出愈率,且以NB培养基为基础配以2,4-D2.0mg/L和6-BA1.0mg/L时,拟南芥的愈伤组织生长状态最好。
The research was to determine the best callus of Arabidopsis thaliana for tissue culture. The callus of Arabidopsis thaliana was induced on the different medium and auxin concentration. The medium NB could improve the callus rate of Arabidopsis thaliana. It was found that the callus was the best on the medium of NB＋2,4-D 2.0mg/L＋6- BA 1.0mg/L.
出处
《河南农业科学》
CSCD
北大核心
2009年第1期42-44,共3页
Journal of Henan Agricultural Sciences
关键词
拟南芥
愈伤组织
激素
培养基
Arabidopsis thaliana
Callus
Auxin
Medium
