摘要
目的:从基因多态性角度探讨冠心病(CHD)痰瘀证的实质。方法:将97例冠心痛患者分为痰证组、瘀证组、痰瘀证组;另设体检健康人群对照组35例。应用聚合酶连反应-限制性片段长度多态性(PCR- RFLP)核苷酸分型技术检测冠心病痰瘀证ApoE的基因型。结果:CHD组与对照组ApoE血清水平比较,差异有非常显著性意义(P<0.01);CHD组各证型ApoE血清水平均明显高于对照组,差异有非常显著性意义(P<0.01);各证型比较,痰瘀证组较其他2证型高,差异有非常显著性意义(P<0.01),瘀证亦高于痰证,差异有显著性意义(P<0.05)。E3/4基因型和E4等位基因频率在痰证患者中明显高于痰瘀证及瘀证患者(P<0.05),而E3/3基因型及E3等位基因在痰瘀证及瘀证患者中的频率则明显高于痰证患者(P<0.05)。结论:ApoE第4外显子的基因型及等位基因频率在冠心病不同中医证候中的分布有明显差异。变异等位基因E4携带者更倾向于发生痰证,表明E4等位基因,特别是E3/4基因型可能是CHD及中医痰证的主要易感基因之一。
Objective:To study the essence of phlegm and blood-stasis syndrome in coronary heart disease (CHD) with the gene polymorphism.Methods:Ninety-seven CHD patients were clarified into phlegm syndrome,blood-stasis syndrome,and phlegm and bland-stasis syndrome. Thirty-five healthy examinees were in the control group.The genotype of ApoE was detected by polymerase chain reaction-restriction fragment length polymorphism (FCB-BFLP) method.Re- sults:The difference of serum ApoE level was significant between the CHD group and the control group (P<0.01).The serum ApoE level was higher in the three syndrome patterns of CHD pa- tients than that in the control group (P<0.01).Among the three patterns,the phlegm and blood-stias syndrome had the highest ApoE level (P<0.01),and blood-stasis syndrome came next (P<0.05).The frequency of E3/4 genotype and E4 allele in the phlegm syndrome was higher than that in the blood-stasis syndrome,and the phlegm and blood-stasis syndrome (P<0.05).In the blood-stasis syndrome,and the phlegm with blood-stria syndrome,the frequen- cy of E3/3 genotype and E3 allele were higher than those in phlegme syndrome(P<0.05).Con- clusion:The frequency of the fourth exon genotype and allele of ApoE varies with the syndrome patterns of CHD,variant E4 allele tends to occur in the phlegm syndrome,indicating that E4 al- lele,in particular E3/4 genotype may he one of the main susceptible genes of CHD and phlegm syndrome.
出处
《新中医》
CAS
北大核心
2009年第2期42-44,共3页
New Chinese Medicine
基金
广东省科技厅课题(编号:2005B33001024)
广东省中医药局课题(编号:2050036)
