核因子-Bp65在口腔白斑及鳞癌中的表达

Expression of NF-B p65 during Oral Leukoplakia Carcinogenesis

目的探讨核因子-Bp65在口腔白斑及鳞癌中的表达。方法应用免疫组织化学技术,检测正常口腔黏膜上皮(n=10),单纯增生型OLK(n=11),异常增生型OLK(n=35),OSCC(n=36)中的NF-Bp65表达情况。结果正常口腔黏膜上皮中p65不表达,由单纯增生型OLK转变到异常增生型OLK最后到OSCC的发生发展过程中p65的表达明显上调。结论p65可能是检测OLK的恶变潜能及OSCC早期诊断的敏感指标。

Objective To investigate the expression of NF - Bp65 in OLK and OSCC,and to evaluate their functions in tumorigenesis and progession of OSCC. Methods Immunohistochemical staining technique was used to determine the expression NF - B p65 in all specimens： the OLK specimens were assessed histopathologically as 11 cases of simple hyperplasia, 35 cases of dysplasia; the OSCC specimens were 36;10 cases of normal oral mucosa（NM） tissue are regarded as normol control. Results The positive rates of NF- B p65 in NM, simple hyperplasia OLK, dysplasia OLK and OSCC were 0% ,9.09% ,42.86% ,and 72. 22% respectively,and a statistical significance was observed among them （ P 〈 0. 05 ）. The strength of p65 protein staining increased with cell proliferation and carcinogenesis, and there were significant differences between the four groups （ P 〈 0. 05 ）. Conclusion NF - B p65 may be useful for evaluating the malignant potential of OLK and early diagnosis of OSCC.