HLA—B51与白塞病相关性研究

Association between HLA-B51 alleles and Behcet＇s disease in Chinese Han nationality

目的探讨HLA—B51等位基因和白塞病的相关性。方法应用PCR—SSP（序列特异性引物）技术对61例中国汉族白塞病患者及100例正常人对照的HLA—B5101-HLA—B5109等位基因进行检测。结果与对照组（11例阳性，11％）相比，白塞病组HLA—B51频率（18例阳性，29．5％）明显增高（r=8．79，P〈0．01，RR=3．39），且白塞病组与对照组中HLA—B51阳性者均为HLA—B5101等位基因。HLA—B51阳性者中男15例，女3例，HLA—B51阴性者中男22例，女21例，两组男女比例差异有统计学意义（P〈0．05）；HLA—B51阳性者平均发病年龄为（28．4±10）岁，HLA—B51阴性者（37.3±12）岁，二者间差异亦有统计学意义（P〈0．05）。在其他各临床表现中，包括类型、病程、皮损、生殖器溃疡、眼部损害、针刺反应、关节受累及皮损类型等均未见到二者差异有统计学意义。结论HLA—B5101等位基因与中国汉族白塞病发病相关，并与患者性别及发病年龄有明显相关性。

Objective To investigate the possible association between HLA-B51 alleles and Behcet＇s disease （BD）. Methods Totally, 61 Chinese patients of Han nationality, who were diagnosed with BD according to the International Study Group （ISG） criteria, were recruited. The control cohort consisted of 100 healthy individuals. Blood samples were obtained from all the subjects. PCR-sequence specific primers （SSPs） were used to for the genotyping of HLA-B51 alleles （HLA-B5101-HLA-B5109）. Results Compared with the control group （11 positive, 11% ）, the frequency of HLA-B51 （18 positive, 29.5% ） was significantly increased in BD patients （x^2 = 8.79, P 〈 0.01, RR = 3.39）. The HLA-B51-positive patients and controls consistently carried HLA-B5101 allele with no other alleles observed. There were 15 males and 3 females in HLA-B51 positive patients, 22 males and 21 females in HLA-B51-negative patients, and significant differences in gender distribution was observed between HLA-B51-positive and negative patients （P 〈 0.05）. Moreover, the average age of onset in HLA-B51-positive patients significantly differed from that in HLA-B51-negative patients （28.4 ± 10 years vs 37.3 ± 12 years, P 〈 0.05）. However, no significant differences were noticed in the clinical types, course, skin lesions, prevalence of genital ulcer, eye damage, joint involvement, or pathergy reaction between HLA-B51-positive and -negative patients （P 〉 0.05）. Conclusions This study supports that HLA-B5101 allele is associated not only with the development of BD, but also with the gender and onset age of patients with BD of Chinese Han nationality.