膀胱尿路上皮癌中LRIG3基因的表达及其临床意义

Expression and Clinical Significanceof the LRIG3 Gene in Urothelial Carcinoma of the Bladder

目的:探讨富含亮氨酸重复序列和免疫球蛋白样多肽3(LRIG3)基因与膀胱尿路上皮癌(BUC)生物学行为的关系。方法:采用免疫组织化学SP法和逆转录-聚合酶链反应(RT-PCR)法检测64例膀胱尿路上皮癌组织和12例正常膀胱黏膜中LRIG3蛋白及mRNA的表达情况,并结合临床资料进行分析。结果:膀胱癌组织中LRIG3蛋白和mRNA的表达水平显著低于正常膀胱黏膜组织,且分级和分期越高,LRIG3蛋白和mRNA的表达水平越低。结论:LRIG3基因在膀胱癌组织中存在表达缺失和低表达,提示LRIG3基因在膀胱癌的发生发展中可能具有抑癌基因的作用,有可能成为肿瘤基因治疗的又一靶点。

Objective：To investigate the relationship between the expression of leucine-rich repeats and immunoglobulin-like domain protein 3 gene （LRIG3）and the biological behavior of bladder urothelial carcinoma （BUC）. Methods： The expressions of LRIG3 in 64 specimens of bladder urothelial carcinoma and 12 specimens of normal bladder tissues were detected by SP of immunohistochemistry and Reverse transcription -- polyrnerase chain reaction method （RT-PCR）, The result s were analyzed in relation to the clinical data. Results： The expression of LRIG3 protein and mRNA in bladder cancer are significantly lower than that of normal bladder epithelium. The expression of LRIG3 protein and mRNA are significantly decreased in higher staging and grading tumors. Conclusions：The expression of LRIG3 gene was lost and reduced in bladder urothelial carcinoma, which indicates that the LRIG3 gene may play an important role in suppressing the tumorigenesis and development in bladder urothelial carcinoma. It may be a new target for tumor gene-therapy.