苦参碱对白色念珠菌黏附人口腔黏膜上皮细胞的影响

Influence of matrine on the adhesion of candida albicans to human buccal epithelial cells

目的:研究不同浓度苦参碱对白色念珠菌粘附人口腔黏膜上皮细胞的影响。方法:采用白色念珠菌标准菌株ATCC76615为研究对象,经不同浓度苦参碱预处理后以及不同浓度苦参碱干预下,在体外和人口腔黏膜上皮细胞相互作用,固定后经革兰氏染色,显微镜下计算粘附细胞数。结果:苦参碱预作用白色念珠菌后,可以减少其对人口腔黏膜上皮细胞的粘附数;苦参碱干预下白色念珠菌粘附人口腔黏膜上皮细胞数明显减少。结论:苦参碱可抑制白色念珠菌粘附人口腔黏膜上皮细胞。

Objective：To investigate the influence of different concentrations of matrine on the adhesion of candida albicans to human buccal epithelial cells. Method： C. albicans collection strain ATCC76615 was obtained and co-cultured with human buccal epithelial cells after following brief exposure to different concentrations of matfine in vitro. The numbers of adherent C. a]bicans were counted in 50 human buccal epithelial cells （BEC） after Grain stain.Result： Compared with the drug-free controls,C, albicans which were pretreated with matrine were seen have a significant reduction in adhension with BECs in vitro. C. albicans which were co-cultured with human BECs in vitro interfered by matrine demonstrated a decreased adhension to BECs.Conclusion： Matrine could inhibit C. albicans adhesion to human BECs in vitro.