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细胞角蛋白在口腔扁平苔藓中表达的电泳研究 被引量：3

The study on alteration of cytokeratins expression in OLP
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摘要 目的:研究口腔扁平苔藓(OLP)中细胞角蛋白(CK)的表达。方法:采用SDS-PAGE、Westernblot法检测15例OLP颊部病变和正常黏膜中细胞角蛋白的表达情况。结果:OLP组与对照组均出现CK1的表达,OLP组中CK2、CK4、CK5表达降低。结论:①.OLP时上皮分化产生CK1使上皮角化增厚,增强颊黏膜的抵抗性。②.OLP中CK2、CK4、CK5的异常表达分别与OLP的异常角化、基底细胞液化坏死有关。 Objective： To explore the possible alteration of cytokeratins expression in OLP and reveal the nosogenesis of OLP. Method：The expression of cytokeratins were detected in 15 samples of OLP by means of SDS-PAGE and Western blot. Result： In control group, we detected the appearance of cytokeratin1,2,4,5,11,13,14,16. There are cytokeratin 1.11.13.14,16,17 in oral buccal mucosa of OLP lesions. Conclusion： （1）. OLP lesions demonstrated profound changes in the site-specific CK profile. The presence of CK1 in OLP lesions may due to a range of stimuli that put a heav- ier functional demand on the tissue. Thus, it can make epithelium thickening. （2）. The decreased expression of CK2,4,5 are respectively correlated with the abnormal keratinization, abnormal differentiation and necrotic alteration of the basal layer.
作者 简洁 聂敏海 冯燕 古亚兰
机构地区 泸州医学院附属口腔医院 四川省德阳市口腔医院
出处 《临床口腔医学杂志》 2009年第1期5-7,共3页 Journal of Clinical Stomatology
基金 四川省教育厅.卫生厅科研项目资助(050093)
关键词 口腔扁平苔藓 细胞角蛋白(CK) SDS—PAGE:Western BLOT lichen planus： western blot SDS-PAGE cytokeratin
分类号 R781.5 [医药卫生—口腔医学]
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参考文献8

  • 1Beverly A Dale, Jukka Salonen, Alma H. Jones.New Approaches and concepts in the study of differentiation of oral epithelia [J]. Oral Biology and Medicine, 1 (3) : 168-190.
  • 2Hanna Mari Pallari,John E Eriksson. Intermediate filaments as signaling platforms[J]. Sci. STKE, 2006, 366: 353.
  • 3Balvinder K, Bloor,Nicholas Tidman,et al. Expression of Keratin K2e in Cutaneous and Oral Lesions [J]. American Journal of Pathology, 2003, 162: 963-975.
  • 4聂敏海,龙汉安,梁尚争,徐晓梅.正常口腔粘膜上皮的细胞角蛋白的表达[J].泸州医学院学报,2001,24(5):380-382. 被引量：4
  • 5Boisnie S, Ouhayoun JP, Branchet MC, et al. Alteration of cytokeratin expression in oral lichen planus [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1995, 79(2): 207-215.
  • 6Bloor BK,Seddon SV, Morgan PR. Gene expression of differentiation-specific keratins (K4,K13,K1 and K10) in oral non-dysplastic keratoses and lichen planus [J]. J Oral Pathol Med, 2000, 29 (8): 376-384.
  • 7毛良军,古亚兰,聂敏海,简洁.细胞角蛋白10、13、14和19在OLP中的表达研究[J].临床口腔医学杂志,2007,23(12):710-712. 被引量：12
  • 8Purkis PE, Steel JB, Mackenzie IC, et al. Antibody markers of basal in complex epithelia [J]. J Cell Sci. 1990, 97: 39-50.

二级参考文献17

  • 1Velden LAVD, Schaafsma HE, Mannii J J, et al. Cytokeratin expression in normal and (pre) malignant head and neck epithelia: an overview [J]. Head & Neck, 1993; 15:133
  • 2Dale BA, Salonen J, Jones AH. New approaches and concepts in the study of oral epithelia [J]. Oral Biol and Med, 1990; 1 (3): 167
  • 3Maeda H. Keratin staining pattern in clinically normal and dis- eased oral mucosa of Lichen planus patients [ J ]. Scand J DensRes, 1994; 102:210
  • 4Branchet MC, Boisnic S, Pascal F, et al. Topical tretinoin inthe treatment of lichen planus and leukoplakia of oral mucosa [J]. A biochemical evaluation of the keratinization. Ann Der-matol Venereol, 1994; 121 (6-7): 464
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  • 6Boisnic S, Frances C, Branchet MC, et al. Immunohistochemical study of oral lesions of lichen planus: diagnostic and pathophysiologic aspects [J]. Oral Surg Oral Med Oral Pathol, 1990, 70 (4) : 462-465.
  • 7Matthews JB, Scully CM, Potts AJ. Oral lichen planus: an immunoper -oxidase study using monoclonal antibodies to lymphocyte subsets [J] .Br J Dermatol, 1984,111 (5) :587-595.
  • 8Debus E,Weber K,Osborn M. Monoclonal cytokeratins antibodies that distinguish simple from stratified squamous epithelia: characterization on human tissue [J]. EMBO J, 1982, 1 (12) : 1641-7.
  • 9Bloor BK,Seddon SV, Morgan PR. Gene expression of differentiation-specific keratins (K4, K13, K1 and K10) in oral non-dysplastic keratoses and lichen planus [J]. Oral Pathol Med, 2000, 29 (8): 376-384.
  • 10Boisnic S, Ouhayoun JP, Branchet MC, et al. Alteration of cytokeratin expression in oral lichen planus [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1995,79 (2) : 207-215.

共引文献11

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临床口腔医学杂志
2009年 第1期

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