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周期性牵张力对人牙周膜细胞MMP-1 mRNA表达的影响 被引量：3

Effects of cyclic-tension force on the expression of MMP-1 mRNA in HPDLF
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摘要 目的:观察周期性牵张应力对人牙周膜细胞(HPDLF)的MMP-1mRNA表达的影响。方法:对培养在弹性膜培养板上的HPDLF施加0.2Hz、12%形变率的周期性牵张力,利用原位杂交和反转录聚合酶链反应(RT-PCR)技术观察HPDLF的MMP-1mRNA的表达。结果:体外培养的HPDLF正常情况下表达MMP-1mRNA,加载周期性牵张力12h、24h、48h后,随着作用时间的延长,HPDLF的MMP-1mRNA有持续增强的趋势。结论:周期性牵张力作用下,HPDLF的MMP-1mRNA的表达增强,加速ECM的代谢活动。 Objective：To investigate the effects of the mechanical strain on the expression of MMP-1 mRNA in HPDLF. Method： HPDLF were subjected to 12% elongation by stretching frequency 0.2Hz in this experiment. The expression of MMP-1 mRNA was determined at 12 h, 24 h, 48 h by in situ hybridization （ISH） and RT-PCR. Result：The expression of MMP-1 was positive in HPDLF cultured in vitro and increased in a time-dependent manner after loading cyclic-ten- sion force. Conclusion： The cyclic-tension force promoted the expression of MMP-I in HPDLF, suggesting possibly important role of MMP-1 in the metabolism of ECM.
作者 张晓东 宋九余 李响 董纪军 李永明 林珠
机构地区 解放军沈阳军区总医院口腔科 第四军医大学口腔医学院正畸科
出处 《临床口腔医学杂志》 2009年第1期16-19,共4页 Journal of Clinical Stomatology
基金 辽宁省博士启动基金资助(20071040)
关键词 牵张应力 牙周膜成纤维细胞 基质金属蛋白酶-1 tensile stress periodontal ligament fibroblast matrix metalloproteinase-1
分类号 Q786 [生物学—分子生物学]
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参考文献9

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二级参考文献2

共引文献6

同被引文献34

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二级引证文献8

临床口腔医学杂志
2009年 第1期

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