蔗糖浓度对变形链球菌wapA基因表达的影响

Effect of concentrations of sucrose on Streptococcus mutans wapA expression

目的:观察不同浓度蔗糖环境对变形链球菌粘附相关基因wapA表达的影响。方法:变形链球菌分别在0.5%、1%、5%蔗糖条件下培养,提取总RNA,逆转录成cDNA,利用TaqMan实时荧光定量PCR技术检测不同环境条件下变形链球菌粘附相关基因wapA的表达。结果:不同浓度蔗糖条件下变形链球菌wapA基因表达的变化不具有统计学意义;0.5%和5%蔗糖条件下,粘附较强的菌株其wapA基因的表达明显高于粘附较弱的菌株。结论:蔗糖量的增加对变形链球菌wapA基因表达的影响不大;wapA基因表达的量可能与变形链球菌的蔗糖依赖性粘附呈正相关关系。

Objective： To detect the influence of concentrations of sucrose on expression of wapA, the adherence associated gene of S. mutans. Method： Total RNA was extracted from S. mutans after being cultured in 0.5%, 1.0%, 5.0% sucrose and then reversely transcripted to cDNA. By utilizing TaqMan RT-RCR technique,we checked wapA expression.Resuit： In different concentrations of sucrose, wapA expression didn＇t changed significantly; In 0.5% and5.0% sucrose,wapA expression of more-adherent S. mutans strains was significantly higher than that of less-adherent strains. Conclusion： The supply of sucrose plays little effect on wapA expression;There might be some correlation between different expression of wapA gene and S. mutans sucrose-dependent adherence.