幼建鲤肌醇缺乏的病理组织学观察及抗氧化能力和肠道菌群变化

Histopathological observation of juvenile Cyprinus carpio var.Jian with myoinositol deficiency and determinations of antioxidative ability and intestinal microbial population

将300尾健康幼建鲤随机分为2组,分别饲喂肌醇含量为163.5 mg/kg(缺乏组)和535.8 mg/kg(对照组)的饲粮,研究了肌醇缺乏对幼建鲤组织器官病理学及抗氧化能力和肠道菌群的影响。结果显示,肌醇缺乏引起幼建鲤皮肤和鳍条糜烂,出现肠绒毛变短和失去正常结构、脾和头肾含铁血黄素沉积、后肾肾小管变形、胰腺酶原颗粒减少、肝细胞空泡变性等病理学改变;抗氧化酶活性和自由基清除能力降低,氧化产物增加(P<0.05);肠道嗜水气单胞菌增加,乳酸菌减少(P<0.05)。表明,肌醇缺乏会造成水生动物组织器官不同程度的病理损伤、抗氧化能力下降和肠道菌群改变。

To determine the histopathological observation and effects of antioxidative capability and intestinal microbial population with dietary myoinositol deficiency, 300 juvenile Cyprinus carpio vat. Jian were randomly distributed into two groups of each three replicates,and was fed with diets containing 163. 5 mg/kg（deficiency group） and 535.8 mg/kg（control group） of myoinositol,respectively. The myoinositol deficiency symptoms was characterized by the damage of skin and fin; intestinal villus were short, ruptured and degeneration of myocardial fiber; deposited siderous heine of spleen and head kidney; degeneration of proximal tubules in hind kidney;the gland pickle reduced and the enzyme granule dissolute in panereas;cytolysis in hepatocytes in the experiment. Meanwhile, myoinositol deficiency induced antioxidative enzyme activities and free radical scavenged ability reduced, and oxidative production increased（P〈0.05）, while the Aeromonas hydrophila significantly increased and Lactobacillus significantly decreased（P（0.05）. The results indicated that myoinositol deficiency induced pathological injuries of tissues,and degraded the antioxidative ability,and inversed microflora in intestine.