3株新城疫病毒体外对人喉癌Hep-2细胞杀伤效应的比较

In Vitro Killing Effects of 3 Newcastle Disease Virus Strains on Human Throat Cancer Hep-2 Cells

目的比较新城疫病毒(NDV)强毒株D817、弱毒株7793和La Sota疫苗株对人喉癌Hep-2细胞的杀伤效应。方法3株病毒经扩增、噬斑纯化后,分别感染Hep-2细胞和喉正常组织细胞,一定时间后,显微镜下观察细胞形态变化,MTT比色法检测病毒对细胞的杀伤效应。结果3株病毒均能在Hep-2细胞中增殖并杀伤细胞,杀伤效应以D817株最高,La Sota株其次,7793株最低,且差异均无统计学意义。杀伤效应与病毒剂量和作用时间呈正相关,显微镜下可见明显的细胞病变。而对喉正常组织细胞的杀伤效应不明显。结论NDV D817株和7793株对喉癌细胞的杀伤效应与La Sota疫苗株相似,而对喉正常组织细胞的杀伤效应不明显,有望成为喉癌生物学治疗新的候选病毒株。

Objective To compare the in vitro killing effects of 3 Newcastle disease virus （NDV） strains on human throat cancer Hep-2 cells. Methods The virulent D817, attenuated 7793 and La Sota strains of NDV were proliferated and purified, then used for infection of Hep-2 cells respectively, using norulal human throat tissue cells as control. The morphological change of Hep-2 cells was observed by microscopy, and the killing effect was determined by MTI＇ method. Results All the 3 virus strains were prolif- erated in Hep-2 cells and killed the cells, while showed no significantly killing effect on normal throat tissue cells. In the turn of killing effect on Hep-2 cells, the 3 strains were D817, La Sota and 7793. However, no significant differences were observed between killing effects of the 3 strains. The killing effect was positively related to the dosage of virus and time for treatment. Obvious CPE of Hep-2 cells was observed by microscopy. Conclusion The killing effects of D817 and 7793 strains of NDV on Hep-2 cells were equivalent to that of La Sota strain. However, neither of the strain showed significantly killing effect on normal throat tissue cells, indicating that D817 and 7793 strains might be used as novel candidate virus strains for biotherapy of throat cancer.