丹参注射液对小鼠鼠尾表皮细胞分化的影响及其作用机制研究

Effects of Salvia Miltiorrhiz Injection on Mouse Tail Epidermal Cell Differentiation and Investigation of the Mechanism

目的探索丹参注射液治疗银屑病的可能机制及量效关系,为临床应用提供实验依据。方法采用小鼠鼠尾鳞片表皮实验模型,不同组别的小鼠以丹参注射液或甲氨喋呤腹腔注射,观察其对鼠尾表皮颗粒细胞生成的作用;计算脾指数、胸腺指数(mg/10g体重);并用ELISA法测定各组小鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)和血清可溶性E-选择素(sE-selectin)水平的变化。结果中、高剂量丹参注射液组和MTX组可显著促进小鼠鼠尾表皮颗粒层细胞的形成并抑制血清TNF-α,IL-2,IFN-γ,sE-selectin的表达,中、高剂量组与MTX组差异无显著性(P>0.05)。低剂量组仅促进表皮颗粒层细胞形成并抑制sE-selectin的表达,与MTX组差异有高度显著性(P<0.01)。各组均对小鼠脾指数、胸腺指数无影响,MTX组可降低小鼠脾指数,显著降低小鼠胸腺指数。结论丹参注射液可能通过阻断多个发病环节起到治疗银屑病的作用,这种作用与剂量有关。

Objective To search possible therapeutic mechanism in treating psoriasis with the Salvia Mihiorrhiz Injection and to explore the correlation between the dosage and therapeutic effects so as to provide experimental basis for its using in clinica. Methods Using mouse tail sealed epidermis as animal experimental mode, different groups of mouse were injected Salvia Miltiorrhiz or methotrexate by tra-abdominally and to Observe the effects of Salvia Miltiorrhiz Injection on mouse tail epidermal cell differ- entiation; calculate mouse spleen index number,thympus index number （mg/10gweigh） ; the level of TNF-α, IL-2, IFN-γ and sE- selectin in mouse serum was measured with enzyme linked immunosordent assay （ELISA） method. Results The middle and high dosage of the Salvia Miltiorrhiz Injection and MTX group could promote greatly the formation of epidermis granular cells on mouse tail and decrease the level of TNF-α, IL-2, IFN-γ, sE-selectin in mouse serum. As compared with that of MTX group, they had no differences（ P 〉 0.05 ）. The low dosage could only promote the formation of epidermis granular cells on mouse tail and to decrease the level of sE-selectin in mouse serum. As compared with that of MTX group, they had significant differences （ P 〈 0.01 ）. SM treated groups had no effect on mouse SIN and TIN. MTX group could decreased mouse＇s SIN and decreased greatly mouse＇s TIN. Conclusion The therapeutic effects of the Salvia Miltiorrhiz Injection on psoriasis might be achieved by blocking several links of chain in the pathogenesis of psoriasis, and these effects were related on dosage.