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RNA干扰沉默Twist基因对人膀胱癌T24细胞增殖的影响

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摘要 目的研究RNA干扰沉默Twist基因对人膀胱癌T24细胞周期和增殖的影响。方法体外构建Twist基因的shRNA表达载体,Li-pofectamin 2000介导转染膀胱癌T24细胞,采用RT-PCR和Western印迹方法检测特异性shRNA对Twist基因沉默效果,转染后采用MTT法检测shR-NA对细胞增殖的作用;应用PI单染流式细胞术分析细胞周期的改变。结果Twist基因的shRNA表达载体有效下调Twist基因表达(P<0.05)。与对照组比较,细胞增殖明显抑制(P<0.05);同时引起细胞G1期阻滞,RNA干扰后,G1期细胞从(48.17±1.62)%增加到(74.34±3.24)%(P<0.05),S期细胞从(33.71±3.54)%减少到(11.58±1.02)%(P<0.05)。结论Twist-shRNA表达载体可以特异高效地抑制膀胱癌T24细胞Twist基因的表达,从而调控肿瘤细胞周期,抑制肿瘤细胞增殖。
出处 《中国老年学杂志》 CAS CSCD 北大核心 2009年第2期155-157,共3页 Chinese Journal of Gerontology
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参考文献8

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