血管紧张素Ⅱ-1型受体(AT1R)基因多态性与OSAHS及OSAHS合并高血压的相关性

Association of the polymorphism of angiotensin Ⅱ type 1 receptor gene with OSAHS and OSAHS complicated with hypertension

目的探讨血管紧张素Ⅱ-1型受体(AT1R)基因A1166/C多态性与阻塞性睡眠呼吸暂停低通气综合征(OSAHS)及其合并高血压的相关性。方法采用聚合酶链反应(PCR)、限制性内切酶酶切及电泳分型方法对OSAHS患者和正常对照人群的AT1R基因A1166/C位点的多态性进行观察,并结合多导睡眠监测结果进行分析。结果正常人群及OSAHS患者的AT1R基因AA、AC、CC型的分布频率分别为88.2%、10.3%、1.5%和72.3%、26.3%、1.4%,两组构成差异有统计学意义(P<0.05)。单纯OSAHS患者及OSAHS合并高血压患者等位基因A和C的频率分别为62%、38%和80%、20%差异显著(P<0.05)。AC及CC基因型的OSAHS患者睡眠呼吸暂停低通气指数(AHI)、平均呼吸暂停时间,最低血氧饱和度均与AA基因型患者差异有统计学意义(P<0.05)。结论OSAHS的发病与AT1R基因A/C多态性相关联,基因型AC和等位基因C可能是OSAHS发病的危险因素。

Objective To investigate the association of angiotensin Ⅱ type 1 receptor （AT1 R） A1166/C polymorphism with obstructive sleep apnea hypopnea syndrome （OSAHS） and OSAHS complicated with hypertension. Methods The polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）was used to investigate the A1166/C polymorphism of AT1R genes of OSAHS patients and healthy subjects. Results The distribution of the AA, AC and CC of AT1R genetypes were 88.2%, 10. 3 % and 1.5 % in the controls while 72. 3% ,26. 3% and 1.4% in clinical OSAHS patients. The variance was significant. The frequencies of A and C allele were 62% and 38% in OSAHS patients and 80% and 20% in OSAHS complicated with hypertension patients. The OSAHS patients with genetype of AC and CC were found to have higher apnea hypopnea index, longer average time of apnea and lower oxygeon saturation comparing to the OSAHS patients with AA genetype of AT1R. Conclusions The polymorphism of AT1R gene is correlated to the OSAHS and OSAHS complicated with hypertension.