survivin蛋白在髓母细胞瘤中的表达及其与预后的关系

Survivin Expression in Medulloblastoma and its Clinical Significance

目的:通过检测髓母细胞瘤(Medulloblastoma)中凋亡抑制蛋白(survivin)的表达情况,探讨它的临床意义,为髓母细胞瘤的治疗寻求新的思路。方法:对50例髓母细胞瘤及10例正常脑组织石蜡切片行免疫组化检测,通过对survivin蛋白在髓母细胞瘤患者与正常脑组织中的表达差异,并运用相关统计分析方法分析它在不同性别、年龄段、肿瘤大小、病理类型、临床分期的表达差异及其与预后的相关性。结果:(1)髓母细胞瘤中survivin蛋白阳性表达率为72%,明显高于正常脑组织的阳性表达率0%,二者表达差异明显(P<0.01);亚组分析发现在survivin蛋白阳性组(n=36)和阴性组(n=14)病例中,性别分布、肿瘤部位、肿瘤大小、病理分型等因素在两者间无明显差别(P>0.05),而年龄及TM临床分期在两组间差异显著(P分别为0.044和0.027)。(2)survivin阳性患者预后与和阴性组相比差异显著(P=0.001)。结论:survivin可能参与了对髓母细胞瘤细胞凋亡的调控,并对髓母细胞瘤的发生发展可能起着重要作用。由于survivin蛋白表达与髓母细胞瘤患者年龄、临床分期及预后等也密切相关,可作为判断病情和评价预后的指标。

Objective： To examine the expression of apoptosis inhibitive protein （survivin） in medulIoblastoma, and in vestigate the clinical significance. To find a new target for the treatment of Medulloblastoma. Methods：Fifty samples of medulloblastoma and 10 samples of normal brain tissue were collected. Immuno histochemistry assay was used to detect the expression of survivin of all samples. The expression of survivin were examined in the groups above. We also com- pared the expression of it according to the sex, age, clinical stage, and size, pathological category of the tumor. And the relationship between the expression and clinical prognosis was analyzed using corresponding statistical methods. Results： （1） Survivin protein was expressed in 36 samples of medulloblastoma （720%）, which remark higher than the control （0%）. There were no differences of gender, position, size and pathological style of tumor between negative and positive of survivin protein groups （P〉0.05） ;but we found that there were different effects of age and clinical stage between the two groups （P= 0. 044 and 0. 027,respectively）;（3）we found that prognosis of survivin-positive patients was different from that of survivin negative patients（P=0. 001）. Conclusion：Our data suggested that survivin was involved in regulation of the apoptosis and cell cycle of medulloblastoma,which indicated that it may play a crucial role in the genesis and progression of medulloblastoma. However,expression level of survivin is releted to patient＇s age and the clinical stage, prognosis of medulloblastoma,and may influence the patients＇ condition and control the prognosis of medulloblastoma.