免疫荧光和免疫组化联合检测难治性癫痫患者脑组织中β类淀粉蛋白的表达

The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination

目的:β淀粉样蛋白(-βamyloid precursor protein,-βAPP)是已知的参与阿尔茨海默病机制的关键因子。-βAPP是否参与难治性癫痫中的病理机制并不清楚。这项研究在于了解-βAPP的蛋白在难治性癫痫患者术后颞叶皮质和海马组织中的表达是否异常。方法:免疫荧光法半定量测定难治性癫痫患者术后颞叶皮质和海马组织中的-βAPP阳性蛋白的荧光值,并应用统计软件对实验数据进行单因素方差分析。结果:免疫荧光强度值分析结果显示-βAPP在耐药性癫痫脑组织中表达较对照组明显增高且有统计学意义。结论:-βAPP在难治性癫痫脑组织中异常增高,增高的-βAPP可能参与了难治性癫痫的病理机制。

Oblective：β-Amyloid precursor protein （β-APP） is known as the key role played in the pathogenesis of Alzheimer＇s disease （AD）. It is now becoming likely that β-APP expression may be associated with refractory epilepsy although the mechanism may not be clear. This study investigated the expression of β- APP protein level in epileptic temporal lobe and hippoeampal tissue compared with human control. Methods：We used immunofluorescence to examine the expression of β-A PP protein levels in the temporal lobes and hippocampus of patients with chronic pharmacoresistant epilepsy. Results：Fluorescence intensity of β-APP protein was shown to be increased significantly in the temporal cortex and hippocampus of the epilepsy group of patients. Conclusions：Over expression of β-APP may contribute to the pathogenetic mechanisms of refractory epilepsy.