摘要
筛选纯化卫矛总黄酮的最佳大孔树脂及其最佳工艺条件,采用正交设计法考察树脂种类、洗脱液浓度、pH值等因素对纯化的影响。用紫外分光光度法测定总黄酮的含量,计算吸附量、解吸率和浸膏总黄酮含量,最后确定最佳工艺条件。AB-8树脂最适于纯化卫矛总黄酮;最佳吸附量和解吸率分别是7.32mg.g-1和90.98%;浸膏的总黄酮含量从7.64%提高到了52.12%。该树脂可以用于精制卫矛总黄酮,提高提取物中总黄酮的含量。
The optimum macroporous resin and process were optimized for the purification of total flavonoids from Euonymus alatus, orthogonal-test method was adopted to study the effects of several factors including the kinds of resins, the concentration of eluent, the pH, etc. , on the purification. The concentration of flavonoids in E. alatus was determined by ultraviolet spectrophotometry, the adsorption capacity, the rate of desorption and the content of total flavonoids in the extract were calculated to determine the suitable conditions. AB-8 resin is suitable for enriching and purifying total flavonoids of E. alatus, the optimum adsorption capacity and the rate of desorption are 7.32 mg·g^-1 and 90.98% respectively. The content of total in the extract was increased from 7. 64% to 52.12%. This resin can be used to enrich and purify the total flavonoids of E. alatus, which can increase the content of total flavonoids in the extract.
出处
《植物研究》
CAS
CSCD
北大核心
2009年第1期124-128,共5页
Bulletin of Botanical Research
基金
黑龙江省科技攻关项目(GC04C50103)
关键词
卫矛
大孔树脂
正交法
纯化
Euonymus alatus
macroporous resin
orthogonal-test
purification
