肺癌患者呼出气冷凝液中基因微卫星改变和p53基因变异的检测及其意义

Significance of detection of chromosome microsatellite alterations and p53 gene mutation in exhaled breath condensate of lung cancer patients

呼出气冷凝液（exhaled breath condensate，EBC）检测是一种新的肺部病变诊断技术。通过检测EBC中相关基因，从而寻找早期诊断肺癌的新方法是目前肺癌研究的热点。采用聚合酶链式反应，对肺癌患者EBC中3号染色体短臂上基因的微卫星异常以及p53基因变异进行检测，结果发现肺癌患者EBC中基因微卫星改变和p53基因变异较正常对照者阳性率明显为高。目前对于EBC基因检测尚属探索阶段，但随着检测技术的不断完善，检测EBC中肺癌的基因标志物是一个较为有希望提高肺癌早期诊断率的方法。

Detection of the exhaled breath condensate（EBC） is a new diagnosis for lung disease. Detecting gene mutation in EBC of lung cancer patients as a potential method of diagnosing lung cancer in early period has become a hot spot in lung cancer research. Some researches,which detect gene mutation in EBC by using polymerase chain reaction, demonstrate that positive rates of p53 gene mutation and chromosome microsatellite alterations in chromosomal region 3p of lung cancer patients are higher than those of healthy peoples. Detecting gene mutation in EBC is still in research stage. However, as the continual development of detecting technique,it is expected to raise the early diagnosis of lung carcinoma.