摘要
试验研究了促卵泡素对山羊卵巢卵泡群生长发育的影响。６只母羊在发情周期的１３～１６ｄ用激素处理，处理前摘取左卵巢，处理后３ｄ摘取右卵巢，组织切片，检测卵泡数。结果显示：当卵母细胞由２层卵泡细胞包围时，开始形成透明带；当达５层以上时，开始形成腔隙。超排和未超排（对照）卵巢上小卵泡数差异不显著（Ｐ＞０．０５），分别为３７２１±１１２个和３６８５±１５６个；而带有５层以上卵泡细胞的卵泡数差异显著（Ｐ＜０．０５），分别为１４０±２０．３个和１１０．０±９．１个；卵泡腔形成后的卵泡数差异非常显著（Ｐ＜０．０１），分别为７５．０±２０．２个和３２．０±１０．１个。带有１～２层卵泡细胞时期的卵泡直径大约３１．６±１．７μｍ，带有３～４层卵泡细胞的卵泡直径大约为５２．３±５．０μｍ，带有５层以上卵泡细胞的卵泡直径为１５１．２±７２．１μｍ，卵泡腔形成后的卵泡直径为０．４～３ｍｍ。
The present study was undertaken to check whether FSH would be effect on ovarian follicular population development during the late luteal and follicular phases. 6 females goat were left ovariectomized at oestrus day 13 before FSH inject. Right ovariectiomize at day 3 after FSH inject. Each right ovary and 8 left ovaries were sectioned at 5 μm and microscopically. The results showed: zona pellucida begin arise, while follicules with 2 or more layers of granulosa cells. Atretic follicular appear, while follicules with 5 layers of granulosa cells. The number of small follicules is no signification difference ( P >0 05) between superovulation and unsuperovulation (3721±112 and 3685±156 respectively). But there is signification ( P<0 05 ) difference the number of follicules with 5 layers of granulosa cells (140±20 3 and 110 0±9 1 respectively). The number of atretic follicules is very signification difference ( P <0 01) 75 0±20 2 and 32 0±10 1 respectively. The diameter of the follicules with 2 or less layers of granulosa cells, with 3￣4 layers of granulosa cells, with 5 or more layers of granulosa cells, and atretic folliculer is 31 6±1 7 μm, 52 3±5 0 μm, 151 2±72 1 μm and 0 4￣3 mm respectively.
出处
《江苏农学院学报》
CSCD
1998年第2期17-20,共4页
Jiangsu Agricultural Research
基金
江苏省教委基金
关键词
山羊
促卵泡素
卵泡
发育
goads
follicle stimulating hormone
graafian follicle
development
