摘要
实验应用ＲＡＰＤ技术，采用集群分离分析方法对小麦白粉病感病品系Ｃｈａｎｃｅｌｏｒ和具有抗性基因Ｐｍ１７的抗病品系Ａｍｉｇｏ及其Ｆ２代抗感集群基因组进行分析，旨在鉴定与抗白粉病基因连锁的分子标记。对３８０个随机引物进行筛选，其结果为：①有４３个引物的扩增片段在亲本间出现多态性；②有两个引物ＯＰＣ０１和ＯＰＤ０３的扩增片段在亲代和Ｆ２代抗感集群中出现一致多态性。
In order to identify molecular marker linked to the resistant gene Pm17 to powdery mildew in Wheat, the genomic DNA of the susceptiable cultivar Chancellor and the resistant line Amigo, as well as four DNA bulks obtainted through Bulked segregant analysis (BSA) were tested in RAPD analysis. After performing RAPD analysis with 380 arbitrary 10 mer primers and agarose gel electrophoresis, 43 of them showed amplified product polymorphisms between two parents. The amplified product polymorphisms of primer OPC01 and OPD03 in parents were consistent with those in segregating F 2 population.
出处
《江苏农学院学报》
CSCD
1998年第2期67-70,共4页
Jiangsu Agricultural Research
基金
"九五"农业部重点课题
江苏省应用基础课题
江苏省青年科技基金
关键词
小麦
白粉病
基因
随机扩增多态性
DNA
抗病性
Triticum aestivum
powdery mildew
genes/random amplified polymorphic DNA(RAPD)
