不同发育期克氏原螯虾卵巢和肝胰腺中孕酮和雌二醇含量变化

Content fluctuations of progesterone and estradiol in the ovary and hepatopancreas of Procambrus clarkia at different developmental stages

对不同发育期的克氏原螯虾的肝胰腺和性腺指数进行了测定,并采用甲醇抽提的方法,利用电化学发光免疫分析仪测定了克氏原螯虾不同发育期的卵巢和肝胰腺中孕酮和雌二醇的含量变化。结果表明,随着卵巢发育,发育初期肝胰腺指数随着发育的进行逐步增加,到初级卵黄发声期到达最大值后随之下降。卵巢中孕酮和雌二醇含量随着卵巢发育的程度而逐步提高,在初级卵黄发生期到达峰值,随后逐渐下降,在肝胰腺中有着相似的趋势,但是峰值出现稍晚,在次级卵黄发生期。

The indexes of gonad and hepatopancreas of P. clarkia at different developmental stages was measured in this paper. The progesterone and estradiol were extracted from the ovary and hepatopanereas of Procambrus clarkia by methanol. The contents were mensurated by the full-automatic electrochemistry luminescence immunity analyzer. The results showed that the indexes of hepatopancreas were increasing in the primary stages of ovary development, and reached its peak in the primary vitellogenesis stage, then descends gradually. The contents of progesterone and estradiol in ovary of P. clarkia were rising along with the development stages the ovary, and the contents of two hormones reach their peaks in primary vitellogenesis stage, and after that both were decreasing. The same trend was also found in the hepatopancreas, the only difference is that the peaks occurred in secondary vitellogenesis stage.