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1个Avr/Cf介导的差异表达基因在番茄抗叶霉病中作用的基因沉默分析

VIGS ANALYSIS OF AN ACE(AVR/CF-ELICITED) GENE IN RESISTANCE TO CLADOSPORIUM FULVUM
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摘要 Avr/Cf介导产生过敏性反应时特异表达的cDNA-AFLP片段,称为ACE(Avr/Cf-elicited)片段,从中挑选1个未知功能的基因(记为ACE5)(GeneBank登录号为CK348288),利用VIGS技术对该片段相应基因进行基因沉默分析,分析该基因在植株生长和发育以及番茄抗叶霉病中的作用。结果表明该差异表达片段相应基因对本氏烟的生长发育以及Avr/Cf介导的HR反应均无影响。 An ACE（Avr/Cf- elicited）gene fragment, ACE5, is a cDNA -AFLP fragment corresponding to a gene specifically expressed during Avr/Cf- dependent hypersensitive response （HR）. ACE5 is chosen as a representative for VIGS analysis to elucidate its possible roles in plant growth and development, and in Avr/Cf- dependent HR. The function of ACE5 is not known. The results show that this gene is not essential for plant growth and Avr/Cf- dependent HR
作者 郑璐平 张志新 徐秋芳 蔡新忠
机构地区 浙江大学农业与生物技术学院生物技术研究所 福建农林大学生物农药与化学生物学教育部重点实验室
出处 《武夷科学》 2008年第1期60-64,共5页 Wuyi Science Journal
基金 福建省教育厅资助项目(JA08079)
关键词 番茄抗叶霉菌基因(cf) 叶霉菌Avr基因 过敏性反应(HR) 病毒诱导的基因沉默(VIGS) cf avr hypersensitive response （HR） virus induced gene silencing （VIGS）.
分类号 S432.2 [农业科学—植物病理学]
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参考文献9

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二级参考文献52

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武夷科学
2008年 第1期

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