摘要
设计一对特异性引物,对小麦矮腥黑粉菌(TCK)及其近似种进行PCR扩增,结果表明TCK可以扩增出200bp清晰条带,而其近似种未出现条带,该方法可以作为TCK的PCR快速检疫鉴定方法。
Dwarf bunt (Tilletia Controversa Kuhn, TCK) is one of the most dangerous quarantine wheat diseases in the world and hardest to control. In this paper, a pair of specific primers were designed and the PCR products showed that only the two TCK samples amplified the 200bp specific band ,the others were not any amplified quick identification of TCK
出处
《武夷科学》
2008年第1期69-72,共4页
Wuyi Science Journal
基金
福建科技重大专项专题(2006NZ0002-1)
福建科技厅自然科学基金项目(X0650003)