福州地区甘蔗宿根矮化病致病菌Leifsonia xyli subsp. Xyli PCR检测

PCR detection for Leifsonia xyli subsp. Xyli,pathogen of the sugarcane ratoon stunting disease

以甘蔗宿根矮化病(ratoon stunting disease,RSD)致病菌Leifsonia xylisubsp.xyli基因组DNA为模板,设计16S与23S基因间隔序列(internal transcript space,ITS)特异PCR引物,建立PCR扩增体系,从福建农林大学甘蔗所资源圃保存的5个甘蔗品种中检测出4个品种感染RSD致病菌。对4个感病甘蔗品种及阳性对照的PCR产物进行测序与分析,结果表明,其序列完全一致,且与NCBI数据库中巴西、澳大利亚分离的RSD致病菌基因组相应区段序列同源性为100%,但与美国路州的有1个碱基的错配和1个碱基的插入。

A pair of specific primers were designed to amplify the internal transcript space region of the 16S--23S ribosomal DNA of Leifsonia xyli subsp, xyli. （Lxx）, pathogen that causes the ratoon stunting disease （RSD）. As a result, a PCR amplification system was established. Four out 5 cultivars planted in the Sugarcane Germplasm Resources Center of the Sugarcane Institute at Fujian Agriculture and Forestry University were infected by the Lxx. The PCR products of the infected plants and a positive control were sequenced and analyzed. The sequences were found to be identical. When compared the NCBI data, they were 100% homologous on the same genome region of DNA with Lxx of Brazil and Australia. On the other hand, there were a mismatched and an insertion base pair in the Lxx as compared with the strain from Louisiana, USA.