EB病毒BHRF1反义寡核苷酸片段诱导鼻咽癌SUNE-1细胞株的凋亡

EBV BHRF1 ANTISENSE OLIGODEOXYNUCLEOTIDE INDUCES APOPTOSIS OF SUNE1 CELL LINE

目的：观察ＥＢ病毒ＢＨＲＦ１反义寡核苷酸片段对鼻咽癌ＳＵＮＥ１细胞株增殖的影响。方法：ＰＣＲ、３ＨＴｄＲ的掺入法及电泳等。结果：ＰＣＲ从ＳＵＮＥ１细胞ＤＮＡ中扩增出ＥＢ病毒的４种（ＢＨＲＦ１、ＤＮＡ酶、胸苷激酶及核抗原１）全基因片段；适当浓度ＢＨＲＦ１反义寡核苷酸片段能引起无血清培养的ＳＵＮＥ１细胞株增殖受抑制；透射电镜及琼脂糖凝胶电泳检测结果发现与ＳＵＮＥ１细胞发生凋亡有关。结论：适当浓度ＢＨＲＦ１反义寡核苷酸片段能抑制无血清培养的ＳＵＮＥ１细胞株的增殖。

Objective:To investigate the effects of EBV BHRF1 antisense oligodeoxynucleotide (ASO) on the proliferation of poorly differentiated nasopharyngeal carcinoma (SUNE1) cell lines. Methods:Polymerase chain reaction (PCR),3HTdR incorporation and electrophoresis. Results:Intact BHRF1, DNase, thymidine kinase (TK) and nuclear antigen1 gene fragments of EBV were amplified using PCR technique with DNAs from B958 and SUNE1 cell lines as template. Inhibited proliferation of SUNE1 was observed in the absence of fetal calf serum(FCS) and exposed to BHRF1ASO. The results from electron microscopy and agarose gel electrophoresis indicated that apoptosis appeared in SUNE[JX*1/9]1 cells. Conclusion:Absence of FCS and exposed to BHRF1ASO on proper concentratin could inhibit proliferation of SUNE1.