香菇135菌株特异SCAR标记的分布和遗传特性

Inheritance Patterns of a Strain-specific Sequence Characterized Amplified Region （SCAR） Marker for Lentinula edodes Strain 135

用香菇(Lentinula edodes)135菌株的特异SCAR(Sequence Characterized Amplified Region)引物135F/R(扩增601bp的条带)对135菌株的58个原生质体单核体和97个孢子单核体的基因组DNA进行扩增.检验此特异标记在单核体中的分布和遗传规律。原生质体单核体分为两种交配型A1B1和A2B2,此标记仅存在于后一种核中;另外,此标记在四种交配型的孢子单核体后代中随机分布,显示这个SCAR标记可以稳定遗传到后代,而且与交配型A、B因子之间没有连锁关系。

PCR amplification employing a SCAR （Sequence Characterized Amplified Region） primer pair, 135F/R, （amplifying a band of 601 bp） for identifying ＇135＇ strains of Lentinula dodes was used to establish the distribution of the specific SCAR marker among 58 protoplast monokaryons and 97 spore monokaryons isolated from a L. edodes 135 strain. Protoplast monok, aryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The marker was also detected in four delineated mating types （A1B1, A2B2, A1B2, A2B1） of spore monokaryons. Our data testify to the stable inheritance of the specific SCAR marker, and show that the SCAR locus is not genetically linked to either the A or B mating factors.