BCG激活淋巴细胞对膀胱肿瘤细胞的抗肿瘤作用研究

Effects of bacillus CalmetteGuérinactivated killer cells against human bladder transitional carcinoma cell line and freshly isolated tumor cells in vitro

利用ＢＣＧ激活的外周血单核细胞（ＰＢＭＮＣ）作为肿瘤杀伤细胞———ＢＡＫ细胞，观察其对移行上皮肿瘤细胞系ＢＴＡ和１１例原代培养细胞的细胞毒作用。结果表明，单独ＢＣＧ对培养肿瘤细胞没有细胞毒作用；ＮＫ细胞仅表现较低的细胞毒作用，在效靶比４０∶１对ＢＴＡ的特异性杀伤率为１７．６３％，对原代细胞为１２．４９％；ＢＡＫ细胞分别为３１．２６％和１９．１６％（Ｐ＜０．０５）；ＬＡＫ细胞分别为３５．５３％和２４．２１％；对ＢＴＡ细胞的细胞毒作用ＬＡＫ细胞＞ＢＡＫ细胞（Ｐ＜０．０５），对原代细胞的细胞毒作用ＬＡＫ细胞和ＢＡＫ细胞之间无显著性差异（Ｐ＞０．０５）。认为ＢＡＫ细胞是不同于ＬＡＫ细胞的一类肿瘤杀伤细胞，在ＢＣＧ抗肿瘤的过程中起着重要作用。

Cytotoxity against human bladder transitional carcinoma cell line BTA and against freshly isolated tumor cells from 11 patients was investigated using bacillus CalmetteGuérinactivated killer cells(BAKcell)from human peripheral blood mononuclear cells(PBMNC). It was found BCG alone was not cytotoxic against the cultured bladder carcinoma cells. These bladder carcinoma cells were also resistant to NK cells. Specific lysis rates at effector/target ratios 40∶1 to BTA was 17.63% and to freshly isolated tumor cells 12.49%. BCG could activate NK cell specific lysis rates against BTA to 31.26% and against freshly isolated tumor cells to 19.16%(P<0.05). The LAK cell specific lysis rates were 35.53% and 24.21% respectively. There was not differences between BAK and LAK cells against freshly isolated tumor cells(P>0.05), but LAK cells were more cytotoxic than BAK cells against BTA cell(P<0.05). The BAK cell might be an important antitumoral mechanism during BCG therapy against superficial urothelial bladder cancer.