补骨脂素体内外抗癌活性的实验研究

An Experimental Study on Antitumor Activity of Psoralen on MammaryCancer Cell Line EMT6 in Vitro and in Vivo

目的：探讨补骨脂素对乳腺癌ＥＭＴ６的抗癌活性。方法：应用ＭＴＴ法、裸鼠移植瘤实验、ＤＮＡ原位定量测定技术及电镜技术，对补骨脂素体内外抑制乳腺癌ＥＭＴ６及肿瘤细胞ＤＮＡ含量变化进行了观察。结果：补骨脂素在体内外对乳腺癌ＥＭＴ６有显著的生长抑制作用，ＩＣ５０为２．２３μｇ／ｍｌ。它可能与细胞ＤＮＡ含量相对减少，细胞线粒体变性，细胞空泡化有关。结论：补骨脂素可能是乳腺癌的有效治疗药物之一。

To investigate the antitumor activity of psoralen on mammary cancer cells of EMT6 line,the cytotoxity of the drug against EMT6 cells was tested by MTT assay;an experimental therapy was carried out in 15 nude mice that were subcutaneously injected with EMT6 cells;the morphological changes of tumor tissues were observed under electron microscope;and the DNA content was measured and analysed with a computerized MPVmicrospectrophotometer.The result shows that psoralen has a strong antitumor effect on mammary cancer EMT6 cells both in vivo and in vitro,with an IC50 value of 2.23μg/ml.The antitumor effect might be related to the relative decrease of cell DNA content and the degeneration and cavitation of cellular mitochondriums.Therefore psoralen may be an effective agent for the treatment of mammary cancer.