补肾方防治地塞米松致雄性大鼠骨质疏松及其生化机制探讨

Study on Efficacy of Chinese Kidney-Tonifying Recipe in Male Rats with Osteoporosis Induced by Dexamethasone and Its Mechanism

目的 :研究补肾方预防和治疗地塞米松致雄性大鼠骨质疏松的疗效 ,并探讨其生化机制。方法 :采用 1 2月龄SD雄性大鼠 46只 ,随机分组给药 ,1 8周后分别测定全身骨密度、股骨抗弯强度以及血清中骨钙素、甲状旁腺素、睾酮、雌二醇的水平。结果 :全身骨密度、股骨抗弯强度预防组显著高于地塞米松组 (P <0 0 1 ) ,与空白对照组比较未见明显差异 ;治疗组高于地塞米松组 (P <0 0 5 ) ,但低于空白对照组 (P <0 0 5 ) ;预防组、治疗组的血清睾酮水平高于地塞米松组 (P <0 0 1和P <0 0 5 ) ,血清甲状旁腺素水平低于地塞米松组 (P <0 0 5 ) ,血清雌二醇以及骨钙素的水平略高于地塞米松组 ,但无统计学意义。结论 :应用补肾方可以有效地预防和治疗地塞米松诱导的雄性大鼠骨质疏松。

Objective: To study the efficacy of traditional Chinese Kidney Tonifying Recipe (KTR) in male rats with osteoporosis induced by dexamethasone (DM) and its mechanism. Methods: Forty six male Sprague Dawley rats at 12 months of age were chosen: they were divided into four groups and KTR and DM were administered appropriately for 18 weeks. General bone mineral density, femur bending strength, serum BGP,serum PTH and serum E 2 were measured. Results: General bone mineral density, femur bending strength in KTR prevention group were significantly higher than those in DM group (P<0 01)and the same with those in normal control group (P>0 05). General bone mineral density, femur bending strength in KTR treatment group were higher than those in DM group (P<0 05), and lower than those in normal control group. Compared with DM group, level of serum T in KTR prevention group and KTR treatment group increased (P<0 05) and level of serum PTH in those two groups decreased (P<0 05). But in comparison with DM group, level of serum E 2 and serum BGP in the above mentioned two groups was unchanged (P>0 05). Conclusions: The data indicated that traditional Chinese Kidney Tonifying Recipe could prevent and treat osteoporosis in male rats induced by DM.