旋覆花中1-氧-乙酰大花旋覆花内酯的分离分析及抑菌活性测定

Separation and analysis of 1-O-acetylbritannilactone from Inula japonica and determination of antifungal activity

为了明确菊科植物旋覆花中主要抑菌活性物质,对旋覆花花序粗提物氯仿萃取组分进行分离,得到化合物1-氧-乙酰大花旋覆花内酯(ABL),运用高效液相色谱法测定花序粗提物中化合物ABL的含量,并测定化合物ABL对黄瓜白粉病菌、黄瓜霜霉病菌、黄瓜灰霉病菌、番茄早疫病菌和致病疫霉等5种植物病原菌的抑菌活性。结果表明,化合物ABL对照品,经紫外光谱、红外光谱、核磁共振等确定结构,纯度为99.5%,符合中药化学对照品含量测定要求。运用高效液相色谱法测定旋覆花花序粗提物中ABL的含量为2.45%,进而计算得到旋覆花花序中ABL的含量约为0.25%。化合物ABL对5种植物病原真菌具有不同的抑菌活性,其中对致病疫霉休止孢萌发抑制作用最强,在浓度为0.1mg/mL时,抑制率可达100%。

In order to clarify the main antifungal compound of Inula japonica, the chloroform extraction of crude extract from I. japonica flowers was separated and compound 1-O-acetylbritannilactone （ABL） was obtained. The content of the compound ABL in flowers crude extract was tested with high performance liquid chromatography. The inhibitory effects of compound ABL against Sphaerotheca fuliginea, Pseudoperonospora cubensis, Botrytis cinerea, Alternaria solani and Phytophthora infestans were tested. The chemical structure of ABL reference substance was elucidated by using ultraviolet spectrometry （UV）, infrared spectroscopy （IR）, nuclear magnetic resonance （NMR）. The purity of ABL reference substance was 99.5%, which satisfies the need of reference substances of traditional Chinese medicines. A method of high performance liquid chromatography was used for the determination of the content of ABL in crude extract from I. japonica flowers （it＇ s 2.45% ）, then the content of ABL in I. japonica was calculated （it＇ s about 0.25% ）. Inhibitory activities of compound ABL against the five phytopathogenic fungi were different, which against the cystospore germination of P. infestans was significant, the inhibition rate was 100% at the concentration 0.1 mg/mL.