山东省首例C群流行性脑脊髓膜炎死亡病例病原学分析

Analysis of First Invasive Meningococcal Disease Death Case Caused by Serogroup C Neisseria Meningitidis in Shandong Province

目的对山东省首例C群流行性脑脊髓膜炎(流脑)死亡病例进行病原学分析。方法对山东省首例C群流脑病例进行流行病学调查,标本采集,菌株分离、鉴定、血清学分群及药物敏感性检测,并进行脉冲场凝胶电泳(Pulse Field Gel Electrophoresis,PFGE)和多位点序列分型(Multilocus Sequence Typing,MLST)分析。结果山东省首次从流脑病人脑脊液中分离到C群脑膜炎奈瑟菌(Neisseria Meningitidis,Nm),该菌株对环丙沙星、左氧沙星、复方新诺明耐药。PFGE分析为AH30型,MLST分析为ST-3200型。结论从山东省首例C群Nm引起死亡病例的脑脊液中分离的菌株,其基因型为ST-3200型,是中国首次发现的C群ST-3200型菌株。应加强对C群Nm的监测,密切关注C群流脑的发病及流行趋势。

Objective To identify and analyze the first Invasive Meningococcal Disease （IMD） Death Case caused by Serogroup C Neisseria meningitidis in Shandong Province. Method CSF specimen from the first suspected IMD serogroupwed resistant to ciprofloxacin, tetracycline, Levofloxacin and trimethoprim-sulfamethoxazole. The strain was PFGE AH30 pattern and ST- 3200. C case was analyzed by culturing, serogrouping, antimicribiol susceptibility test, Pulse field gel electrophoresis （PFGE） and Multilocus sequence typing （MLST）. Result The first sero- group C Neisseria meningitidis strain from CSF of patient was isolated and confirmed in Shandong province. Conclusion The first death IMV case caused by serogroup C Neisseria rniningitidis was confirmed. The first ST-3200 serogroup C strain was found firstly in China,differing from the serogroup C strains causing outbreaks in Anhui province. Surveillance on serogroup C epidemic and outbreaks should be strengthened in Shandong province.