急性重复性缺氧时原代培养脑细胞中白介素-6基因表达

The changes of IL-6 gene expression in acute repetitive hypoxia

目的:探讨急性重复性缺氧时IL-6变化的意义。方法:将胎脑制成原代培养的脑细胞,活细胞率在95%以上时,通入95%N_2+5%CO_23分钟,再通入95%O_2+50%CO_210分钟,重复上述过程一次。应用逆转录一多聚合酶链式反应(RT-PCR)检测缺氧适应后人原代培养的脑细胞白介素-6(IL-6)的基因表达的变化,用计算机凝胶成像及分析系统对PCR产物进行分析。结果:(1)第一次缺氧后IL-6基因表达最强,第二次缺氧后表达减弱,但还强于对照组;(2)RNA无明显降解。结论:在缺氧适应时IL-6参与了细胞保护作用。

Objective: This study was to observe the changes of IL-6 gene expression in acute repetitive hypoxia. Method: When the ratio of living cells was more than 95%, nerve cells cultured were passed through with mixed gases of 95% N_2+5%CO_2 for 3 min followed by another gases mixture of 95% O2+5% CO_2 for 10 min. After repeated the experiment as above, the alteration in the expression of IL-6 gene was measured using the RT-PCR method. The prod ucts of PCR were analyzed with computer Gel imaging and image analysis instrument. Result: After the first hypoxia IL-6 gene expression enhanced, and after the second hypoxia, it was still stronger than baseline although it declined slightly. There was no significant RNA disintegration. Conclusion:The IL-6 involves in the cellular defense in hypoxia adaptation.