冠心病血瘀证ApoE基因多态性的检测分析

Detective analysis on polymorphism of apolipoprotein E gene in blood-stasis syndrome of coronary heart disease

目的探讨载脂蛋白E(ApoE)基因多态性与冠心病心血瘀阻证及血脂指标的相关关系。方法对冠心病心血瘀阻证组112例、冠心病非心血瘀阻证组108例、非冠心病心血瘀阻证组110例和健康人对照组100例检测分析ApoE基因型(E2/E3、E2/E4、E3/E3、E3/E4、E4/E4)和等位基因(ε2、ε3、ε4)及血脂指标(TC、TG、HDL-C、LDL-C)。结果冠心病心血瘀证组的ε4等位基因和E3/E4基因型的频率明显高于健康人对照组(均P<0.05和<0.01);而冠心病非心血瘀证组、非冠心病心血瘀证组在基因型和基因频率分布上与健康人对照组比较均无显著性差异;TC、LDL-C两项指标均呈冠心病心血瘀证>非冠心病血瘀证>冠心病非血瘀证>健康人对照组递减的趋势,而HDL-C相反,则呈递升的趋势;TC、TG、LDL-C等3项指标与ApoE-E3/E4都存在着相关关系(均P<0.01),但ApoE-E3/E4与这4项指标都有差异,其阳性率不同(均P<0.01)。结论提示冠心病心血瘀阻证与ε4等位基因和E3/E4基因型及血脂异常均有一定关系;TC、TG、LDL-C等3项指标与ApoE-E3/E4都存在着相关关系(均P<0.01)。ApoE基因多态性可能是促使冠心病和血瘀证发生的遗传因素。

Objective To investigate the relationship between the polymorphism of apolipoprotein E （ApoE） and heart blood-stasis syndrome of coronary heart disease （CHD） or the indexes of blood lipid. Method The types of ApoE （E2/E3, E2/E4, E3/E3, E3/E4, E4/E4）, alleles （ε2, ε3, ε4） and indexes of blood lipid （ TC, TG, HDL-C, LDL-C ） were detected and analyzed in the group of heart blood-stasis syndrome of CHD （group 1, n = 112）, CHD group （group 2, n = 108 ）, group of heart blood-stasis syndrome （ group 3, n = 110） and healthy control group （ group 4, n = 100）. Result In group 1 the frequencies of allele ε4 and genotype of E3/E4 were much higher than those in group 4 （ all P 〈0.05 and all P 〈0.01 ）. There were no significant differences in genotype and frequency distribution between group 2 or 3 and group 4. The indexes of TC and LDL-C had a degressive trend showed as that in group 1 〉 in group 3 〉 in group 2 〉 in group 4, while the index of HDL-C, on the contrary, had a progressive trend. The indexes of TC, TG and LDL-C were correlated to ApoE-E3/E4 （ all P 〈 0.01 ）, but ApoE-E3/FA was different in positive rate from the indexes of TC, TG, HDL-C and LDL-C （ all P 〈 0.01 ）. Conclusion The heart blood-stasis syndrome of CHD is related to allele ε4, genotype of E3/E4 and abnormal blood lipid. There is a correlation between the indexes of TC, TG or LDL-C and ApoE-E3/ FA （all P 〈 0.01 ）. The polymorphism of ApoE may be the hereditary factor of CHD and blood-stasis syndrome.