摘要
研究了细菌阿特拉津氯水解酶基因atzA在转基因水稻AA269株系自交和回交后代中的遗传规律.结果表明:atzA基因作为一个显性遗传位点遵循孟德尔遗传分离规律.除草剂抗性鉴定结果表明,在T_1代水稻群体中除草剂抗性和敏感株数遵循3:1的分离规律,T_2代稳定纯合;T_2～T_7自交世代的转基因植株均保持稳定的除草剂抗性.在BC_1～BC_3代群体中除草剂抗性和敏感株数遵循1:1的分离规律,BC群体自交的BCF_2群体中遵循3:1分离规律.分别对AA269/9311的B_3F_1和B_2F_2群体中的一个株系进行了PCR分析,PCR阳性株与阴性株的分离比例分别符合1:1和3:1,与除草剂抗性鉴定结果一致.对T_7代群体中部分单株的Southern杂交和RT-PCR分析表明,atzA基因已稳定遗传至T_7代,并得到表达.
The genetic rule of bacterial atrazine chlorohydrolase gene atzA in self-cross and back-cross progenies of transgenic rice ＂AA269＂ were investigated. The results showed that as a dominant genetic locus,atzA gene followed Mendel＇s genetic segregation rule. The herbicideresistance identification results showed that herbicide-resistance plants and herbicide-sensitivity plants followed 3：1 segregation rule in T1,and stably homozygotic in T2 and herbicide-resistance had been stably inherited to T7,and BC1-BC3 and BCF2 （self-cross of BC）also followed 1：1 and 3：1 segregation rules respectively. The segregation ratio of PCR positive plants and PCR negative plants of BC3F1 and BC2F2 followed 1 ： 1 and 3 ： 1 segregation rule. The PCR results are identical to that of herbicide-resistance identification. The Southern blot hybridization and RTPCR analysis showed that atzA gene had been stably inherited to T7 generation and expressed steadily in T7.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期99-102,共4页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津农学院科技发展基金(2004013)
